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Volume 6 Supplement 6

Beyond the Genome 2012

  • Poster presentation
  • Open Access

Massively parallel sequencing of formalin-fixed paraffin-embedded tissues

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BMC Proceedings20126 (Suppl 6) :P40

https://doi.org/10.1186/1753-6561-6-S6-P40

  • Published:

Keywords

  • Public Health
  • Nucleic Acid
  • Paraffin
  • Common Method
  • Parallel Sequencing

Formalin fixation followed by paraffin embedment (FFPE) is the most common method of preserving resected tissues. FFPEs can be easily stored, retrieved and processed for further analysis when compared to the logistical complications of processing fresh frozen (FF) material. Conversely, they may not be ideal for sequencing because of the nucleic acid fragmentation and artifacts introduced by fixation. Here we report our efforts in performing next-generation sequencing on more than 70 specimens comprising >40 FFPE samples, from low to high quality, and compared the performance of FFPE, FF and commercially available cell lines.

Authors’ Affiliations

(1)
Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA

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