Figure 1

HDMEC microvasculature in the MOC device. a) Exploded view of the device comprising a polycarbonate CP (blue), the PDMS-glass chip accommodating two microvascular circuits (yellow; footprint: 76 mm × 25 mm; height: 3 mm) and a heatable MOC-holder (red). b) Calcein AM assay (red) showed viable and evenly distributed HDMEC in all areas of the circulation. Scale bar = 2 mm. c) Image stack taken by two-photon laser scanning microscopy. HDMEC were able to cover all walls of the channels forming a fluid tight layer. Functionality of the established microvascular vessel system was demonstrated by d) ac-LDL uptake of HDMEC and e) CD31 (red), vWF (green) expression throughout the entire cell population. Nuclei were counterstained with Hoechst 33342 (blue). Scale bar = 100μm.