Table 1 Calculated efficiencies (E), Cq and ΔCq values and copies relative to ß-actin for gDNA and cDNA derived from clones 3D6-IgA and 4B3-IgA

ß-actin

gDNA

3D6-IgA

24.60

0.20

2.07

2.22

n/a

n/a

4B3-IgA

24.21

0.14

2.07

2.22

n/a

n/a

cDNA

3D6-IgA

18.52

0.13

2.03

0.43

n/a

n/a

4B3-IgA

16.25

0.63

2.04

1.33

n/a

n/a

HC

gDNA

3D6-IgA

23.56

0.16

1.95

3.32

-1.03

8.28

4B3-IgA

22.11

0.14

1.95

3.32

-2.11

16.44

cDNA

3D6-IgA

21.78

0.17

1.91

1.35

3.26

0.38

4B3-IgA

19.50

0.68

1.97

1.53

3.25

0.20

JC

gDNA

3D6-IgA

24.81

0.03

1.95

0.94

0.22

3.80

4B3-IgA

22.77

0.10

1.95

0.94

-1.44

11.20

cDNA

3D6-IgA

24.52

0.23

1.82

0.87

5.97

0.22

4B3-IgA

20.81

1.54

1.96

0.27

4.56

0.10

LC

gDNA

3D6-IgA

24.90

0.14

2.05

0.59

0.31

0.98

4B3-IgA

21.50

0.21

2.11

1.21

-2.71

4.40

cDNA

3D6-IgA

20.26

0.20

1.88

0.75

1.73

1.30

4B3-IgA

15.02

2.36

1.98

1.30

-1.22

3.93