NIR-spectroscopy for bioprocess monitoring & control

BMC Proceedings

Table 1 NIR results for calibration models and validation by external data sets.

Analyte	Range	No. Cal. No. Val. Batches (Samples)		Reg. maths	Factors	SEC	SEP
TCC (·10⁶ cell/mL)	0-16	5 (185)	3 (118)	None	2	1.07	0.48
Viability (%)	10-100	5 (193)	3 (110)	None	4	4.2	4.2
Glucose (g/L)	0-9	5 (198)	3 (105)	None	4	1.2	0.48
Glutamine (g/L)	0-1.1	5 (189)	3 (114)	SNV	2	0.16	correlation

(TCC: total cell count; No.Cal.: Number of batches (samples) of the calibration set. No.Val.: Number of batches (samples) of the validation set; SNV: standard normal variate; SEC: standard error of calibration; SEP: standard error of prediction)

ISSN: 1753-6561