Table 1 Comparison between the fluorescence-based quantitation method and the p24 ELISA assay
From: Characterization and quantitation of fluorescent Gag virus-like particles
Fluorescence-based method | p24 ELISA assay | |
|---|---|---|
Specificity | Gag-GFP fusion protein | Gag-GFP fusion protein |
Linear range | 7 to 1000 RFU (10 to 3600 ng of p24/mL) | 10 to 300 pg of p24/mL |
Precision | ~2% CV | ~10% CV |
Limit of detection | 10 ng/mL of p24 | 10 pg/mL of p24 |
Time (96 samples) | ~1.5 h | ~4 h |
Price (96 samples) | ~10 € | ~400 € |