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Figure 1 | BMC Proceedings

Figure 1

From: Development of 3D human intestinal equivalents for substance testing in microliter-scale on a multi-organ-chip

Figure 1

a-d) Characterization of the decellularization procedure. a) Explanted jejunal segment with the preserved capillary bed after decellularization. b) H/E staining of the decellularized matrix. c) Feulgen staining of the decellularized matrix. d) immunofluorescent stainings for collagen I on rBioVaSc. e) The multi-organ-chip (MOC) device consisting of an integrated micro-pump, a microfluidic .channel system and inserts for the cultivation of different organ equivalents. f+g) Characterization of the intestinal in vitro equivalent. f) H/E staining of the recellularized matrix after one week of dynamic culture in the MOC device. g) Second Harmonic Generation by 2 PM, nuceli were stained with Hoechst 33342.

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