Table 1 Comparison of fed-batch cultures
From: Highly efficient inoculum propagation in perfusion culture using WAVE Bioreactor™ systems
FB seeded from batch | FB seeded from perfusion | |
|---|---|---|
Cell conc. at cell removal [c/mL] | 2.2 × 106 | 2.3 × 107 |
Split ratio | 1:5 | 1:30 |
Inoculum conc. [c/mL] | 4.1 × 105 | 7.4 × 105 |
Process time [d] | 14 | 14 |
Peak cell conc. [c/mL] | 1.4 × 107 | 1.7 × 107 |
Av. μ during growth phase [d-1] | 0.44 | 0.52 |