Table 1 Central composite face-centered result
From: Optimized fermentation conditions for improved antibody yield in hybridoma cells
Exp No | FBS [%] | Pluronic [g/L] | IGF [ug/L) | Viability [%] | Viable cell concentration [cells/mL] |
|---|---|---|---|---|---|
1 | -1 (1) | -1 (0.2) | -1 (10) | 75.8 | 736000 |
2 | 1 (10) | -1 (0.2) | -1 (10) | 79.1 | 1.468e+006 |
3 | -1 (1) | 1 (1) | -1 (10) | 66.6 | 575000 |
4 | 1 (10) | 1 (1) | -1 (10) | 82 | 1.401e+006 |
5 | -1 (1) | -1 (0.2) | 1 (100) | 71.6 | 696000 |
6 | 1 (10) | -1 (0.2) | 1 (100) | 77.9 | 1.545e+006 |
7 | -1 (1) | 1 (1) | 1 (100) | 59.3 | 554000 |
8 | 1 (10) | 1 (1) | 1 (100) | 78.7 | 1.319e+006 |
9 | -1 (1) | 0 (0.6) | 0 (55) | 69.1 | 632000 |
10 | 1 (10) | 0 (0.6) | 0 (55) | 79.8 | 1.455e+006 |
11 | 0 (5.5) | -1 (0.2) | 0 (55) | 82.5 | 1.461e+006 |
12 | 0 (5.5) | 1 (1) | 0 (55) | 78.9 | 1.442e+006 |
13 | 0 (5.5) | 0 (0.6) | -1 (10) | 79.1 | 1.326e+006 |
14 | 0 (5.5) | 0 (0.6) | 1 (100) | 81.6 | 1.336e+006 |
15 | 0 (5.5) | 0 (0.6) | 0 (55) | 81.8 | 1.27e+006 |
16 | 0 (5.5) | 0 (0.6) | 0 (55) | 80.2 | 1.194e+006 |
17 | 0 (5.5) | 0 (0.6) | 0 (55) | 81.3 | 1.188e+006 |
18 | 0 | 0.6 | 55 | 28.5 | 13300 |
19 | 5.5 | 0 | 55 | 78.4 | 1.255e+006 |
20 | 5.5 | 0.6 | 0 | 81.25 | 1.2685e+006 |
21 | 5.5 | 0.2 | 100 | 83.7 | 1.533e+006 |
22 | 10 | 0 | 0 | 83.6 | 1.28e+006 |
23 | 6 | 0 | 0 | 81.9 | 1.305e+006 |
24 | 1 | 0 | 0 | 57.2 | 464000 |