Figure 1
From: 2D fluorescence spectroscopy for real-time aggregation monitoring in upstream processing
PCA score plots for all Bis-ANS (A), thiovlavin T (B) and ANS (C) concentrations, where PC2 is displayed over PC1. T = 0 indicates data of 2D scans taken directly after inoculation. (D) SEC chromatogram of the intrinsic fluorescence emission signal at 355nm. Monomer, dimer and oligomer fractions of mAb were detectable; furthermore a separation from the medium and host cell protein signal was possible.