Table 3 Primer Design Rule Parameters
From: Better primer design for metagenomics applications by increasing taxonomic distinguishability
Design Parameters | Values Used |
|---|---|
allowable 3' ends | S[SW,WS][SW,WS,SS] |
allowable 5' ends | WW, [WS,SW]SW |
MINTEMP | 48 |
MAXTEMP | 70 |
MAXTEMPRANGE | 8 |
MINCG% | 40 |
MAXCG% | 60 |
MINPRIMERLENGTH | 17 |
MAXPRIMERLENGTH | 24 |
MAXDEGENERACY | 64 |
MAXSELFDIMER | 6 |
MAXPRIMERDIMER | 6 |
MAXRUNLENGTH (bp) | 4 |
MINAMPLICONLENGTH | 150 |
MAXAMPLICONLENGTH | 600 |