Table 5 Comparison of Primer Pairs
From: Better primer design for metagenomics applications by increasing taxonomic distinguishability
Primer Pair Parameters | Total Number of Hits per Taxon | Number of Distinguishable Amplicons per Taxon | |||||||
|---|---|---|---|---|---|---|---|---|---|
PrimerPairID | TmRange | Dimer | Strain | Species | Genus | Phylum | Species | Genus | Phylum |
F345-R1052 | 6.83 | 4 | 8738 | 7961 | 1702 | 29 | 7110 | 1696 | 29 |
F345-R505 | 5.69 | 4 | 8654 | 7887 | 1684 | 27 | 4726 | 1554 | 27 |
F345-R775 | 5.18 | 4 | 7981 | 7342 | 1565 | 24 | 5943 | 1549 | 24 |
F345-R873 | 6.47 | 5 | 7961 | 7264 | 1537 | 27 | 6031 | 1527 | 27 |
F345-R922 | 6.83 | 5 | 7740 | 7074 | 1452 | 22 | 5893 | 1443 | 22 |
F528-R1052 | 6.83 | 4 | 8212 | 7520 | 1647 | 27 | 6295 | 1639 | 27 |
F797-R1052 | 6.83 | 3 | 8399 | 7720 | 1639 | 25 | 5396 | 1610 | 25 |
F1064-R1367 | 6.48 | 4 | 8155 | 7439 | 1596 | 27 | 4926 | 1534 | 27 |
F1185-R1367 | 7.58 | 4 | 8099 | 7375 | 1568 | 28 | 3776 | 1407 | 28 |
HMPV35 | 9.67 | 5 | 8174 | 7452 | 1573 | 24 | 6225 | 1564 | 24 |
HMPV13 | 0.79 | 3 | 1572 | 1198 | 498 | 18 | 1104 | 497 | 18 |
HMPV69 | 7.38 | 3 | 1162 | 818 | 304 | 20 | 646 | 301 | 20 |
U337F-805R | 10.48 | 3 | 7011 | 6479 | 1405 | 17 | 5218 | 1392 | 17 |
U337F-518R | 4.81 | 6 | 7059 | 6512 | 1429 | 20 | 3886 | 1325 | 20 |
NossaV34 | 6.47 | 4 | 8042 | 7413 | 1546 | 17 | 5997 | 1533 | 17 |