Highlighted mutations with important impact on residue interactions. A comparison network is shown in Cytoscape (left) and a visualization of the aligned structures (scTIM in gray, dTIM in red) in UCSF Chimera (right). In the network view, green dashed edges depict gained, and red dotted edges lost interactions. The network nodes are colored according to the fraction of adjacent interaction edges that do not change upon mutation (from white for all to gray for none), the node border colors represent the conservation score of the respective residue in the parent with turquoise-to-pink coloring for variable-to-conserved sites. Nodes with an amino acid mutation are shown as diamonds. The mutated residues with the largest impact on the residue interactions are highlighted by yellow colored nodes in the network views and by green boundaries and the ball-and-stick representations in the structure view. The mutations correspond to the following residue pairs based on the alignment of scTIM (chain A) and dTIM sequences: (A30, -), (S31, K30), (I32, L31), (E34, D33), (N35, D34), (L68, K67), (N78, I77), (K89, D88), (S100, H99), (V154, L153), (-, E156).