Feature 1: Extreme conservation of sequences displaying asparagine in Gram-negatives in position 13. Highlighting all AKL domain sequences in Gram-negative bacteria displaying asparagine (N) in position 13 exposes extreme conservation of the selected sequences throughout the length of the visualised protein. The total number of highlighted sequences is 48, including 46 identical (minor variation occurs only in two sequences in positions 2, 3, 6, 12, 14, 15, 20, 21, 23, 26, 30, 33 and 35). The underlying causes for this unusual conservation remain to be investigated. Due to the scale of this extreme conservation (nearly 5.4% of the whole Gram-negative dataset), the findings can have significant implications for the interpretation and evaluation of data.