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In vitro anthelmintic activity of Pothomorphe umbellata (L.) Miq. (Piperaceae) against gastrointestinal parasites from sheep

  • Luis Eduardo Ferreira1,
  • Pedro Castro1,
  • Franca Suzelei1 and
  • Beleboni Rene1
BMC Proceedings20148(Suppl 4):P155

Published: 1 October 2014


Leaf ExtractAdult WormParasitic NematodeInfective LarvaAdult Form

Sheep farming in Brazil has been proved to be a promising economical activity by the offering of products with increased interest. Infections with gastrointestinal parasites from family of Trichostrongylidae, especially represented by Haemonchus contortus, are one the most limiting problem in sheep production, aggravated by the increasing resistance of nematodes to traditional anthelmintic drugs. Ethnopharmacological data have indicated the Pothomorphe umbellata as a promising alternative for the control of gastrointestinal nematodes. The aim of this work was to evaluate the in vitro anthelmintic effects of P. umbellata aqueous leaf extract against eggs, infective larvae and adult forms of parasitic nematodes from Trichostrongylidae family from sheep. At higher doses, P. umbellata extract showed 95.82%, and 32.18% of efficacy in egg hatch test (EHT) and larval motility test (LMT), respectively. In the adult worm motility test, worms were completely immobilized within the first 4-6 hours of nematode exposition to different dilutions of extract. Thus, these data showed that the evaluated extract presented significant anthelmintic activity, which are of high biotechnological interest and useful towards the growth of sheep farming in Brazil.

Authors’ Affiliations

Unit of Biotechnology, Ribeirão Preto University (UNAERP), Ribeirão Preto, Brazil


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© Ferreira et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.