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BMC Proceedings

Open Access

Culture optimization of Escherichia coli for expression of gE proteinfrombovine herpesvirus 1 and 5

  • Maraninchi Roberta Silveira1,
  • Cláudia Pinho Hartleben1,
  • Leonardo Garcia Monte1,
  • Gizele Lima de Sá1,
  • Fabrício Conceição2,
  • Fabrício Souza Campos3,
  • Paulo Roehe3 and
  • Bianca Sica Siedler4
BMC Proceedings20148(Suppl 4):P167

https://doi.org/10.1186/1753-6561-8-S4-P167

Published: 1 October 2014

Background

The use of Escherichia coli for the production of recombinant proteins is an established strategy to obtain biotechnological tools. However, the recombinant protein expression is dependent on temperature, bacterial culture time, induction period, nutrients, plasmid characteristics, and insert itself. Thus, the aim of this study was optimize the expression of recombinant gE protein (rgE) from Bovine Herpesvirus types 1 and 5.

Methods

The expression at various post-induction time-points and during growth at two temperatures was performed in order to standardize the conditions that E. coli maximizes rgE expression. A recombinant vector pAE/gE containing a consensus sequence between BoHV-1 and BoHV-5 was used to heat-shock transform E. coli strain BL21 Star™ (DE3). The transformation product was seeded on solid Luria Bertani (LB) medium containing ampicillin (100 µg/mL). After, the colonies selected were grown in LB medium (1 mL) and incubated at 37 °C for 16 h. Then, 0.5 mL of this culture was transferred to 10 mL of LB medium and incubated at 37 °C again to reach the exponential phase of bacterial growth (OD600 0.6 - 0.8). The bacterial culture was induced with 0.6 mM IPTG for periods of 4, 6 and 12 h at 25 ºC or 37 ºC. Aliquots from each culture condition tested were collected and analyzed by SDS-PAGE and Western Blot (WB).

Results and conclusions

The finding of this study indicated that rgE protein was successfully expressed after induction for 12 h at 25 °C. These conditions will be used to obtain rgE lots for development of immunodiagnostic assays of bovine Herpesvirus type 1 and 5.

Declarations

Acknowledgements

We want to thanks to Universidade Federal de Pelotas, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) and Cnpq (Conselho Nacional de Desenvolvimento Científico e Tecnológico).

Authors’ Affiliations

(1)
Centro de Desenvolvimento Tecnológico, Biotecnologia, Laboratório de Imunodiagnóstico, Universidade Federal de Pelotas
(2)
Universidade Federal de Pelotas
(3)
Instituto de Biociências, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul
(4)
Centro de Desenvolvimento Tecnológico, Biotecnologia, Laboratório de Imunodiagnóstico, Universidade Federal de Pelotas

References

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  3. Delhon G, Moraes MP, Lu Z, Afonso CL, Flores EF, Weiblen R, Kutish GF, Rock DL: Genomeofbovineherpesvirus 5. Journal of Virology. 2003, 77 (19): 10339-10347. 10.1128/JVI.77.19.10339-10347.2003.PubMed CentralView ArticlePubMedGoogle Scholar
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Copyright

© Silveira et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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