Molecular characterization of strains of Zymomonas mobilis by sequencing the 16S ribosomal
© Araujo et al.; licensee BioMed Central Ltd. 2014
Published: 1 October 2014
Zymomonasmobilis has attracted great interest in the scientific, industrial and biotechnological due to its high potential fermentation. From the viewpoint of taxonomic Z. mobilis is the only species of the genus Zymomonas , and is subdivided into three subspecies: Z. mobilis subsp. mobilis, Z. mobilis subsp. pomaceae and Z. mobilis susp. francensis. Differentiation between these three subspecies is based on physiological tests. These tests are time consuming and often unreliable. Therefore, molecular techniques are proposed as a quick and reliable way to characterize the genetic variability of these bacteria. This study aimed to perform molecular characterization of 6 strains of Zymomonasmobilis deposited in the Collection of Microorganisms of Department of Antibiotics, Federal University of Pernambuco (UFPEDA).
The strains were grown in SSDL for 24 hours at 30 °, followed by centrifugation and extraction of chromosomal DNA. PCR reactions were performed using specific primers and conditions for amplification of the 16S rDNA. The products of the amplified 16S rDNA were purified and sequenced using a ABI PRISM® 3500 Genetic Analyzer (Applied Biosystems) at the Sequencing Platform LABCEN/CCB in the Universidade Federal de Pernambuco (Recife, Brazil). The data obtained by sequencing the 16S rDNA were analyzed and compared by BLASTn programs and aligned by MultiAlin.
Results and conclusion
The sequences obtained were different degrees of similarity to strains from international collections. Multiple alignment of the sequences of the 16S rDNA of strains of Z. mobilis UFPEDA gene regions revealed a high degree of conservation. Sequence analysis of the 16S rDNA confirmed that all the strains belong to the species Zymomonas mobilis . However, it was not possible to differentiate the level of subspecies. From the results obtained by multiple alignment of the sequences was possible to prove the stability and degree of conservation of all lineages. Based on these results, other phylogenetic markers should be used to better characterize the genetic variability of strains of Z. mobilis deposited at the collection UFPEDA.
The Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support and the Sequencing Platform LABCEN/CCB in the Universidade Federal de Pernambuco (Recife, Brazil).
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