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BMC Proceedings

Open Access

BUL: a novel lectin from Bauhinia ungulata L. seeds with fungistatic and antiproliferative activities

  • André Silva1,
  • Talita Leite1,
  • Edson Teixeira1,
  • Luiz Ponte2 and
  • Luciano Pinto3
BMC Proceedings20148(Suppl 4):P87

https://doi.org/10.1186/1753-6561-8-S4-P87

Published: 1 October 2014

A new galactose-binding lectin, termed BUL, has been purified from seeds of Bauhinia ungulata (Caesalpinoideae) by precipitation with solid ammonium sulfate followed by agarose-lactose affinity chromatography. B. ungulata lectin strongly agglutinated rabbit erythrocytes, both native and treated with proteolytic enzymes, and was inhibited by D-galactose and D-galactose-derived sugars, especially N-acetyl-D-galactosamine. BUL was shown to be a stable glycoprotein, maintaining its hemagglutinating activity after incubation at wide ranges of temperature and pH, but not after incubation with EDTA. By SDS-PAGE analysis under reduced conditions, purified BUL showed an electrophoretic profile consisting of a single band with apparent molecular mass of 30 kDa. BUL showed intrinsic fluorescence typical of folded globular proteins, and circular dichroism spectra of lectin in the native state showed a predominance of β-sheet secondary structure. The N-terminal amino acid sequence of 19 residues showed a high sequential similarity to other galactose-specific lectins from the Bauhinia genus. In addition, BUL showed antifungal activity against phytopathogenic species and showed in vitro antiproliferative activity against the HT-29 cell line of human colon adenocarcinoma in a dose-dependent manner.

Authors’ Affiliations

(1)
Universidade Federal do Ceará
(2)
Universidade Estadual do Vale do Acaraú
(3)
Universidade Federal de Pelotas

Copyright

© Silva et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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