Figure 1
From: Front-line of genome editing technology for animal cell engineering
The PITCh systems for gene knock-in mediated by MMEJ. The original PITCh systems, TAL-PITCh and CRIS-PITCh (v1), utilize proximal MMEJ (P-MMEJ) (a, b), whereas the modified PITCh system, CRIS-PITCh (v2), utilizes distal MMEJ (D-MMEJ) (c, d). PITCh systems enable both whole plasmid integration (a, c) and particular cassette integration without carrying the plasmid backbone sequence (b, d). Red and blue boxes indicate the microhomologous sequence.