Figure 2
From: Adventitious agent contamination risk mitigation: engineering MMV virus resistance into CHO cells
(a) Schematic of the qPCR based assay for MMV virus attachment, internalization, and single cycle replication. In brief the virus is incubated with the cells at an MOI of 0.1 for 3 hours. Samples are washed 3 times to remove excess unbound virus (T = 0) and harvested after an additional 21 hrs and analyzed by qPCR for presence of viral genome copies. (b) Viral genome copies compared to the WT at times T = 0 and T = 21 for the COSMC- and the Slc35A1- knock out clones, and (c) for the St3Gal4 -/- and the St3Gal4-/- + St3Gal6-/- knock out clones.