Figure 1
From: Expression of glycoproteins with excellent glycosylation profile and serum half-life in CAP-Go cells
Expression of recombinant human C1 Inh in CAP-Go.2 cells results in glycoproteins with increased sialylation of N-glycans, homogenous core 1 O-glycosylation and significant increased serum half-life. A) Recombinant proteins were produced using the indicated platform, purified and subjected to isolelectric focusing (IEF) gel analysis in comparison to plasma-derived Berinert. B) ECL Lectin Blot, comparing degree of sialylation between different CAP-Go cell lines and hC1 Inh from human serum (Berinert). Erythrina cristagalli lectin binds to terminal galactose on N-glycans, sialic acid prevents the lectin from binding, therefore decrease signal intensity indicates increase in sialylation. C) MALDI-TOF mass spectrum analysis of C1 Inh O-glycans of protein expressed in CAP-Go.1 or CAP-Go.2 cell lines in comparison to Berinert. D and E) C1 Inh preparations were injected intravenously at 10 mg/kg into female Sprague Dawley rats. Residual C1 in plasma samples taken at the indicated time points was analyzed by ELISA.