Cell Line: | Industrial suspension CHO producing mAb biosimilar |
Seed: | 0.3 - 0.5 × 106 viable cells/mL in 5 L CDFortiCHO (Life Technologies) |
Expansion: | Expand at 2 - 4 viable cells/mL to 14 L |
Feeding strategy: | CD EfficientFeed C (EFC, Life Technologies), initial feed at 8.0 ± 2.0 M viable cells.mL Additional bolus feeds every 24 hours for a total of 5 feeds (1.2 L/feed) |
Carbon source: | Glucose kept above >2 f/L (bolus target >5f/L) |
Antifoam C: | Added as needed |
Aeration rate: | 1.0 L/min |
DO set point: | O2 control at 40% |
pH set point: | 7.2 ± 0.05 |
Agitation: | 5 L: 25 RPM, 5°X, 5°Y; 14 - 20: 30 or 35 RPM, 15°X, 5°Y |
Temperature: | 37°C |