Figure 1

Viable cell density and viability (1), caspase 9 activity (2) and PCA of ICM MS biotyping (3). Batch (a) and perfusion mode (b). VCD: viable cell density; FC RLU: fold change of relative luminescence units; PC: principal component of the respective analysis. (1) closed symbols, VCD and open symbols, viability (3) each dot represents one technical replicate (up to eight technical replicates for one biological sample). Grey rectangle in B (1) and (2) indicates the time span of continuous medium exchange. Dashed lines illustrate at which point culture alteration is detectable with respective method.