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Table 1 Expansion of aMSCs with various microcarriers in static or agitated small scale platforms.

From: Media and microcarrier surface must be optimized when transitioning mesenchymal stem/stromal cell expansion to stirred tank bioreactors

 

Animal origin-free

Day 7 fold increase

% Recovery

% Viability

Overall cell yield (E+04 cells / mL)

Rank

  

Petri

Spinner

Petri

Spinner

Petri

Spinner

Petri

Spinner

 

Microcarrier Aa

No

24

24

35

52

98

95

4.5

10.3

3

Microcarrier Ba

Yes

28

15

46

58

92

91

9.2

8.3

2

Microcarrier Ca

Yes

21

16

59

86

98

93

11.6

18.8

1

Microcarrier D

Yes

28

12

25

84

97

95

2.9

13.5

4

Microcarrier E

Yes

29

16

27

46

96

97

3.3

5.3

5

Microcarrier F

No

24

17

22

30

91

90

1.9

2.4

6

  1. aMicrocarriers chosen for further evaluation in a bioreactor process.