Figure 1
From: Dynamic cultivation of human stem cells under physiological conditions
MSC cultivated on a 3D ceramic scaffold for 21 days under static and dynamic perfusion conditions with 21% and 5% O 2 in standard growth medium (GM) or osteogenic differentiation medium (ODM). (1) MTT-viability assay. (2) Glucose consumption and lactate formation. (3) Matrix deposition: (A) calcein staining for extracellular calcium, (B) alicarin red staining for extracellular calcium, (C) Von Kossa staining for extracellular phosphate.