- Poster presentation
- Open Access
Borna disease virus phosphoprotein interferes with neuronal function and contributes to neurobehavioral disorders
© Prat et al; licensee BioMed Central Ltd. 2008
- Published: 23 September 2008
- Synaptic Activity
- Microelectrode Array
- Primary Neuronal Culture
- Synaptic Potentiation
- Infected Neuron
Infection by Borna disease virus (BDV) enables the study of the molecular mechanisms whereby a virus can persist in the central nervous system and lead to altered brain function, in the absence of overt cytolysis and inflammation. This neurotropic virus infects a wide variety of vertebrates and causes behavioral diseases. The basis of BDV-induced behavioral impairment remains largely unknown.
Previously, we have shown that BDV specifically blocks the activity-dependent enhancement of synaptic activity, both by studying the recycling of synaptic vesicles and by using electrophysiological approaches on BDV-infected neuronal networks grown on microelectrode arrays. This suggested defects in long-term potentiation, one key component of learning at the cellular level. Studies of signaling pathways involved in synaptic potentiation revealed that this blockade was due to an interference with PKC-dependent signaling in neurons, likely due to the viral phosphoprotein (P).
Here, we used recombinant BDV with mutated PKC phosphorylation sites on P , and showed that this mutation restored the phosphorylation of PKC substrates in neurons after stimulation. Moreover, using primary neuronal cultures grown on micro-electrode arrays (MEA), we provide evidence that the activity-dependent enhancement of synaptic activity was restored when cultures were infected with the P-mutated virus. Therefore, preventing P protein phosphorylation by PKC completely restores normal neuronal activity upon stimulation in infected neurons.
Together, these findings illustrate a novel mechanism whereby a viral protein can cause synaptic dysfunction and contribute to neurobehavioral disorders.
This article is published under license to BioMed Central Ltd.