Estimating genomic breeding values from the QTL-MAS Workshop Data using a single SNP and haplotype/IBD approach
© Calus et al; licensee BioMed Central Ltd. 2009
Published: 23 February 2009
Genomic breeding values were estimated using a Gibbs sampler that avoided the use of the Metropolis-Hastings step as implemented in the BayesB model of Meuwissen et al., Genetics 2001, 157:1819–1829.
Two models that estimated genomic estimated breeding values (EBVs) were applied: one used constructed haplotypes (based on alleles of 20 markers) and IBD matrices, another used single SNP regression. Both models were applied with or without polygenic effect. A fifth model included only polygenic effects and no genomic information.
The models needed to estimate 366,959 effects for the haplotype/IBD approach, but only 11,850 effects for the single SNP approach. The four genomic models identified 11 to 14 regions that had a posterior QTL probability >0.1. Accuracies of genomic selection breeding values for animals in generations 4–6 ranged from 0.84 to 0.87 (haplotype/IBD vs. SNP).
It can be concluded that including a polygenic effect in the genomic model had no effect on the accuracy of the total EBVs or prediction of the QTL positions. The SNP model yielded slightly higher accuracies for the total EBVs, while both models were able to detect nearly all QTL that explained at least 0.5% of the total phenotypic variance.
The applied models to estimate genomic breeding values described in this paper, are derived from a multiple QTL mapping model described by Meuwissen and Goddard . The methods are implemented using variable (i.e. in this case presence of a QTL or not on a putative QTL position) selection via Gibbs sampling . Thus, the applied Bayesian method avoids the computationally costly Metropolis-Hastings step that was implemented in the BayesB model of Meuwissen et al. .
Parameterization of the model
where y i is the phenotype of animal i, μ is the overall mean, s i is a fixed effect for sexe, u i is the polygenic effect of animal i, v j is the direction of the QTL effects of the haplotypes at putative QTL position j, qij1 (qij2) is the size of the QTL effect for the paternal (maternal) haplotype of animal i at putative QTL position j, and e i is the residual term for animal i . Note that the total effect of a haplotype is modeled as q ij. × v j , and that q ij . and v j may have a positive or negative value. The covariance among polygenic effects (u.) was modeled as A × , where A is the relationship matrix which was based on the full pedigree and is the polygenic variance. The second model (called 'HAP_NOPOL') was the same as HAP_POL, but omitted the polygenic component. The HAP models assumed a putative QTL in the midpoint of each marker bracket. The covariances among haplotypes at bracket j (q.j.) were modeled as H j , which is the matrix of estimated IBD probabilities among the haplotypes at the midpoint of bracket j. The variance of q.j .was assumed 1, while v j is a scale parameter that accommodates for a bracket to have a large (small) effect, if a QTL is (not) present. IBD probabilities between haplotypes were calculated using the algorithm of Meuwissen and Goddard , which combines linkage disequilibrium with linkage information and, for each bracket j, considers 20 surrounding markers and all available pedigree information. The effective population size was assumed 100 and the number of generations since an arbitrary founder population was also assumed 100, as in Meuwissen and Goddard . All pairs of base haplotypes (i.e haplotypes of first generation of genotyped animals) with an IBD probability above 0.95 were clustered, using a hierarchical clustering algorithm. If the matrix of IBD probabilities among base haplotypes was not positive definite after clustering, the matrix was bended by adding |min_eigenval| + 0.01 to all the diagonal elements, where |min_eigenval| is the absolute value of the lowest (negative) eigenvalue. The matrix was subsequently inverted by LU denomposition. The elements in for the descendant haplotypes were then calculated using the algorithm of Fernando and Grossman (1989) . When the IBD probability of descendant haplotypes with one of their parental haplotypes exceeded 0.95, the descendant haplotype was clustered with this parental haplotype.
where y i , s i , μ, and u i are as in model 1, v j is the direction of the effects of the alleles at marker locus j, qij1 and qij2 are the sizes of the marker effects of animal i at marker locus j, and e i is the residual term for animal i. The fourth model (called 'SNP_NOPOL') was the same as SNP_POL, but omitted the polygenic component.
For reasons of comparison, a fifth model was applied, which did include the polygenic effects, but omitted the SNP effects. This model was called 'POL'.
For all models, a Markov chain Monte Carlo method using Gibbs sampling was used to obtain posterior estimates for all the effects in the model . The scale parameter of a putative QTL at locus j, v j , was sampled from a normal distribution N(0, ), if a QTL was present in bracket j, whereas v j was sampled from N(0, /100) if no QTL was not present in bracket j. The variance of v j , , was sampled from an scaled inverse chi-square distribution with a prior variance of 0.058. This prior variance was calculated as the additive genetic variance, estimated using model 'POL', divided by 30, i.e. assuming 30 additive and unrelated QTL affecting the trait, across the 6 chromosomes. The presence of a QTL in bracket j was sampled from a Bernoulli distribution with probability equal to , where P(v j |) is the probability of sampling v j from N(0, ), i.e. , and Prj is prior probability of the presence of a QTL in bracket j. Prj was calculated per bracket as five times (i.e. assuming five QTL per chromosome) the length of bracket j, divided by the total length of all the brackets on the chromosome. More details on the prior distributions and the fully conditional distributions can be found in Meuwissen and Goddard . The Gibbs sampler was implemented using residual updating, which was proven to be an computationally efficient way to solve the equations . The Gibbs sampler was run for all models for 30,000 iterations and 3,000 iterations were removed as burn-in.
Correlations (reflecting accuracy) between true and estimated breeding values, and coefficients of regression of true breeding values on estimated breeding values (estimated using all five models) for animals without phenotypes in generations 4, 5 and 6.
Correlations between phenotypes and estimated breeding values for animals with phenotypes, estimated using all five models.
Correlations between estimated breeding values for animals without phenotypes, estimated using all five models.
The presented methods have been applied in multiple studies, where they proved to be able to detect QTL [1, 7] as well as estimate genomic breeding values accurately [7–9]. In the present study, differences in accuracies of the EBVs of the HAP and SNP models were small, which is in agreement with the finding that for r2 values between adjacent markers of ~0.2 the differences in accuracies of the HAP and SNP models are negligible . Apparently, including linkage analysis information next to linkage disequilibrium information in the model (i.e. going from the SNP to the HAP model), does not yield additional information to estimate effects more accurately.
Interestingly, the POL model yielded a higher correlation between EBV and phenotype than the genomic models. However, the accuracy of the EBVs for animals with the genomic models were 0.93–0.94, while the accuracy for the same animals were only 0.70 for the POL model (results not shown).
For the provided data set, including a polygenic effect in the genomic model had no effect on the accuracy of the total EBVs or prediction of the QTL positions. The SNP model yielded slightly higher accuracies for the total EBVs, while both models were able to detect nearly all QTL that explained at least 0.5% of the total phenotypic variance.
Hendrix Genetics, CRV B.V., and NWO-Casimir (The Netherlands Organization for Scientific Research) are acknowledged for financial support. The authors thank John Bastiaansen for suggestions and comments on the results of this study.
This article has been published as part of BMC Proceedings Volume 3 Supplement 1, 2009: Proceedings of the 12th European workshop on QTL mapping and marker assisted selection. The full contents of the supplement are available online at http://www.biomedcentral.com/1753-6561/3?issue=S1.
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