MGBG–cisplatin combination chemotherapy against breast cancer
© Gonsalves et al; licensee BioMed Central Ltd. 2010
Published: 24 September 2010
Worldwide, it is estimated that more than one million women are diagnosed with breast cancer every year, representing 14% of female cancer deaths , about 4,500 new cases being detected each year in Portugal. In the present study, the compound metylglyoxal bis(guanylhydrazone) (MGBG) , an inhibitor of S-adenosyl-L-methionine descarboxylase (SAMdc), was investigated as a potencial anti-cancer agent towards the breast cancer cell line MCF-7 and the non-carcinogenic, non-immortalized, human foreskin fibroblast cells BJ. The results (MTT assay) evidenced that the effect of MGBG against the MCF-7 cells is dose and time dependent, revealing a significant cell viability loss (ca. 90% at 72 h, for a 50 μM dosage). Since its effect was not shown to be reversible, this compound appears to be quite effective in this line. In fact, compared to cisplatin (cDDP), a commonly used drug in clinical practice, MGBG provided a larger cytotoxicity in the same concentration range. Similarly, a slight synergistic effect was verified for these two compounds – ca. 93% at 72 h, for an MGBG(50 μM):CDDP(10 μM) combination, while no effect was assessed on the reversibility of the cytotoxic action. Regarding the non-neoplastic BJ line, MBGB exhibited a clear reversibility of et growth-inhibiting effect, as opposed to cDDP and the MGBG:cDDP cocktails.
- Coughlin SS, Ekwueme DU: Breast cancer as a global health concern. Cancer Epidemiol. 2009, 33: 315-318. 10.1016/j.canep.2009.10.003.View ArticlePubMedGoogle Scholar
- Marques MPM, Gil FPSC, Calheiros R, Battaglia V, Brunati AM, Agostinelli E, Toninello A: Biological activity of antitumoural MGBG: the structural variable. Amino Acids. 2008, 34: 555-564. 10.1007/s00726-007-0009-2.View ArticlePubMedGoogle Scholar
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