Cytotoxic effect induced by combination of polyamines metabolites and endocannabinoid, anandamide, on human cancer cells: a new anticancer strategy
© Batista-de-Carvalho et al; licensee BioMed Central Ltd. 2010
Published: 24 September 2010
The sensitizing effect was also greater on multidrug-resistant cells than wild-type ones, an aspect of particular importance since conventional cancer therapy suffers from the development of drug resistance. Cell viability was determined using MTT assay . Concentrations 0-100 µM of AEA were tested, for incubation times up to 24 h; and concentrations 0-8 µM of spermine in presence of BSAO were used, for incubation times up to 1 h.
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