Micropropagation of Pinus taedaL. via axillary buds
© Francisdo de Oliveira et al; licensee BioMed Central Ltd. 2011
Published: 13 September 2011
Pinus taeda stands for productivity and quality of its timber . Researches using biotechnology are of great importance and have been applied to the improvement of its timber and plantation . The main method of Pinus propagation is by seeds, once the minicuttings depends on the season of the year or depends of juvenile material [3–5]. Thus, researches on micropropagation of Pinus taeda are currently a priority in Brazil . Micropropagation is the best method for mass production of superior genotypes and represents a strategy for tree improvement and capture of genetic gains . Studies on Pinus taeda micropropagation by axillary bud proliferation are quite few. The purpose of this study was to develop a protocol for micropropagation of this species from juvenile material.
Materials and methods
For in vitro establishment two to four month old seedlings were used. Apical shoots and nodal segments of 3 cm length were inoculated in MS , DCR , WV3  or WV5  medium. For axillary shoots induction, the explants were inoculated in WV3, WV5 or DCR medium, with BAP (0, 0.12, 0.25 and 0.50 μM). For the induction of roots, we tested the effect of double-layer medium, with semi-solid phase consisting of agar and water or GDm/2  medium and the liquid phase containing water or GDm/2 medium. Both phases were supplemented with 2.69 μM NAA and 0.44 μM BAP for 9 days, followed by transfer to growth regulator-free GDm/2 medium. The rooted plants were planted in Plantmax® Forestry substrate and maintained in a greenhouse.
Results and Discussion
The autors are grateful to Battistella Florestal Company for the financial assistance, to FINEP (Financiadora de Estudos e Projetos), to REUNI (Reestruturação e Expansão das Universidades Federais) and the Federal University of Paraná, Brazil.
- Schultz RI: Genetics and tree improvement. Loblolly pine: the ecology and culture of loblolly pine (Pinus taeda L.). Edited by: Schultz RI. New Orleans. 1997, U.S. Department of Agriculture, Forest Service, 1-50.Google Scholar
- Gonzalez-Benecke CA, Martin TA, Clark III A, Peter GF: Water availability and genetic effects on wood properties of loblolly pine (Pinus taeda). Canadian Journal of Forest Research. 2010, 40: 2265-2277. 10.1139/X10-162.View ArticleGoogle Scholar
- Stojičić D, Budimir S, Ćulafić L: Micropropagation of Pinus heldreichii. Plant Cell, Tissue and Organ Culture. 1999, 59: 147-150. 10.1023/A:1006373218772.View ArticleGoogle Scholar
- Alcantara GB, Ribas LLF, Higa AR, Ribas KCZ, Koehler HS: Efeito da idade da muda e da estação do ano no enraizamento de miniestacas de Pinus taeda L. Revista Árvore. 2007, 31: 399-404.View ArticleGoogle Scholar
- Andrejow GMP, Higa AR: Potencial de enraizamento de miniestacas de Pinus taeda L. provenientes de brotação apical de mudas jovens. Floresta. 2009, 39: 897-903.View ArticleGoogle Scholar
- Faleiros M: Plantação a partir de clones de pínus já é feita no Brazil. 2011, Available in http://www.revistaopapel.org.br/noticia-anexos/1272649863_aee1535dc2037f6433ca5e0011dcd609_138912915.pdfGoogle Scholar
- Gupta PK, Durzan DJ: Loblolly pine (Pinus taeda L.). Biotechnology in Agriculture and Forestry Trees III. Edited by: Bajaj YPS. 1991, Verlag Berlin Heidelberg New York: Springer, 16: 383-407.Google Scholar
- Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962, 15: 473-497. 10.1111/j.1399-3054.1962.tb08052.x.View ArticleGoogle Scholar
- Gupta PK, Durzan DJ: Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Reports. 1985, 4: 177-179. 10.1007/BF00269282.View ArticlePubMedGoogle Scholar
- Coke JE: Basal nutrient medium for in vitro cultures of loblolly pines. USA Patent 5.534.434. 1996, Available in http://www.freepatentsonline.com/5534434.pdf accessed in May 16 2011Google Scholar
- Coke JE: Basal nutrient medium for in vitro cultures of loblolly pines. USA Patent 5.534.434. 1996, Available in http://www.freepatentsonline.com/5534433.pdf accessed in May 16 2011,Google Scholar
- Mehra-Palta A, Smeltzer RH, Mott RL: Hormonal control of induced organogenesis experiments with excised plant parts of loblolly pine. Tappi Journal. 1978, 61: 37-40.Google Scholar
- Chang S, Sen S, McKinley CR, Aimers-Halliday J, Newton RJ: Clonal propagation of Virginia Pine (Pinus virginiana Mill.) by organogenesis. Plant Cell Reports. 1991, 10: 131-134.View ArticlePubMedGoogle Scholar
- Leach GN: Growth in soil of plantlets produced by tissue culture. Tappi Journal. 1979, 62: 59-61.Google Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.