Volume 5 Supplement 7
Gravitropic response in radiata pine seedlings. Searching molecular keys
© Ramos et al; licensee BioMed Central Ltd. 2011
Published: 13 September 2011
One-year old Pinus radiata seedlings were inclined during 15 days (d) and transversally cut at 5d, 8d and 15d. Stem slices were stained using a solution of phloroglucinol (2A). From these preparations, wall thickness (2B) and cell diameter (2C) of xylem cells were measured.
This project was supported by FONDECYT (1071026), DIAT (U. de Talca), PBCT Anillo (ACT-41), Ecos-Conicyt (CB07-01) and postdoctoral PBCT-PSD61 projects. P.R. thanks to Conicyt for doctoral fellowship.
- Larson PR, Kretschmann DE, Clark A, Isebrands JG: Formation and properties of juvenile wood in southern pines: a synopsis. Gen. Tech. Rep. FPL-GTR-129. 2001, Madison, WI: U.S. Department of Agriculture, Forest Service, Forest Products Laboratory, 42-Google Scholar
- Diatchenko L, Lau Y-FC, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD: Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. P Natl Acad Sci, USA. 1996, 93: 6025-6030. 10.1073/pnas.93.12.6025.View ArticleGoogle Scholar
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