Genetic diversity of Bertholletia excelsa, an Amazonian species of wide distribution
© Sujii et al; licensee BioMed Central Ltd. 2011
Published: 13 September 2011
Amazonian upland forests are expansive and can comprise large continuous tracts. There have been several studies on the population genetic structure of species in this kind of forest, but there are few studies that aim to understand genetic structure throughout the Amazon . The Brazil-nut tree, Bertholletia excelsa, is a monotypic genus, endemic to upland forests and distributed along almost the entire expanse of the Amazon [2–5].
Genetic diversity distribution through Amazon forest is an understudied issue, specially in plants. Quantifying and understanding population genetic structure, associated to gene flow and mating system studies are recognized as important tools for the development of strategies for conservation and management. Such information can also be helpful in identifying effects of habitat fragmentation [6, 7].
This study aimed to evaluate genetic structure of B. excelsa populations in the Amazon and to verify if the structuring is influenced by distance between them.
Seven polymorphic microsatellite markers were developed from a dinucleotide-enriched genomic library. Material from 379 individuals was collected from nine Amazonian subpopulations distributed in five Brazilian states (AC: Acre, AM: Amazonas, AP: Amapá, PA: Pará, RR: Roraima). All seven microsatellite markers developed for the species were used with four others previously published markers , for genotyping.
Analyses within and among populations were performed to evaluate genetic diversity and population structure. All nine populations were characterized with 11 microsatellite loci for number of alleles per locus, allele frequency and observed and expected heterozygosity under Hardy-Weinberg expectation. Wright fixation indices were also estimated. Genetic distance estimates were correlated with different potential factors to find possible causes of genetic structure.
Results and conclusions
A few alleles were found in each subpopulation and considerable variation was observed in alleles found in each subpopulation and in their allele frequencies, especially when compared with very distant subpopulations. Heterozygote excess was observed in five subpopulations while in the other four subpopulations, estimates of F IS , non-significantly different from zero, were found. The observed high heterozygote proportions in most subpopulations and absence of inbreeding support the existence of self-incompatibility mechanisms and selection in favor of heterozygotes. Maintenance of genetic variability is favored by the alogamous breeding system, which is an important feature to be considered in conservation and management strategies, in order to avoid fertility deficits.
Fine-scale structure, when present, was small. Estimates of inter population genetic structure varied from low (q= 0,02) to high (q= 0,244). B. excelsa genetic structure can be analyzed on three different scales: (i) within population; (ii) among moderately distant populations (<500km); and (iii) among very distant subpopulations. At all scales, significant correlations were found between genetic structure and geographic distance between pairs of individuals or populations. This may indicate that distance is an important factor in this population’s genetic structure, but probably there are other factors acting in conjunction, especially on a large geographical scale.
- Sampaio AJ: A flora amazônica. Revista Brazileira de Geografia. 1942, 4 (2): 57-72.Google Scholar
- Maués MM: Reprodutive phenology and pollination of the Brazil nut tree (Bertholletia excelsa Humb. & Bonpl., Lecythidaceae) in Eastern Amazonia. Pollinating Bees The Conservation Link Between Agriculture and Nature - Ministry of Environment / Brazilia. Edited by: P. KEVAN & V.L. Imperatriz Fonseca. 2002, 245-254.Google Scholar
- Mori SA, Prance GT: Taxonomy, ecology and economic botany of the Brazil nut (Bertholletia excelsa Humb. & Bonpl. Lecythidaceae). Advances in Economic Botany. 1990, 8: 130-150.Google Scholar
- Moritz A: Estudos biológicos da floração e frutificação da castanha-do-Brazil. DocumentosEmbrapa/Cpatu, Belém. 1984, 29: 1-78.Google Scholar
- Salomão R: Densidade, estrutura e distribuição espacial de castanheira-do-Brazil (Bertholletia excelsa H. & B.) em dois platôs de floresta ombrófila densa na Amazônia setentrional Brazileira. Boletim do Museu Paraense Emílio Goeldi. 2009, 4: 11-25.Google Scholar
- Azevedo VCR, Kanashiro M, Ciampi AY, Grattapaglia : Genetic structure and mating system of Manilkara huberi (Ducke) A. Chev., a heavily logged Amazonian timber species. Journal of Heredity. 2007, 98: 646-654. 10.1093/jhered/esm074.View ArticlePubMedGoogle Scholar
- Erickson DL, Hamrick JL, Kochert GD: Ecological determinants of genetic diversity in an expanding population of the shrub Myrica cerifera. Molecular Ecology. 2004, 13: 1655-1664. 10.1111/j.1365-294X.2004.02139.x.View ArticlePubMedGoogle Scholar
- Reis AMM, Braga AC, Lemes MM, Gribel R, Collevatti RG: Development and characterization of microsatellite markers for the Brazil nut tree Bertholletia excels Humb & Bonpl. (Lecythidaceae). Molecular Ecology Resources. 2009, 9: 920-923. 10.1111/j.1755-0998.2008.02481.x.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.