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  • Open Access

Apparent coordination of isocitrate dehydrogenase and glutamate decarboxylase expression in early stages of tree development

  • 1,
  • 2,
  • 3,
  • 2 and
  • 2Email author
BMC Proceedings20115 (Suppl 7) :P66

  • Published:


  • Nitrogen
  • Glutamate
  • Tree Species
  • Developmental Process
  • Plant Development

The biosynthesis of 2-oxoglutarate and glutamate are key steps in the biosynthesis of nitrogen compounds and plant development. The reaction catalyzed by cytosolic isoenzyme of NADP+-linked isocitrate dehydrogenase (IDH) is also considered as the main route in the production of 2-oxoglutarate. According to its expression pattern during development, IDH is also involved in other, yet unknown, processes [1, 2]. In addition to the importance of glutamate in the biosynthesis of nitrogen compounds, glutamate also serves as precursor of GABA, a molecule that is currently considered as a signal in higher plants. GABA is produced by the action of glutamate decarboxylase (GAD), a cytosolic enzyme that is regulated by Ca2+/calmodulin and pH. In contrast to IDH, that it is encoded by just one gene in most of plant genomes [2], GAD is encoded by a small family of nuclear genes [3]. The expression of IDH and GAD has been investigated during the differentiation of hypocotyl and stem in tree species. Our results indicate a coordination of the expression of IDH and GAD in developmental processes suggesting a role for 2-oxoglutarate supply and GABA synthesis during early stages of organ differentiation in trees.

Authors’ Affiliations

Present address: Rutgers University, Dpt Biological Sciences, Newark, New Jersey, USA
Universidad de Málaga, Dpt. Biología Molecular y Bioquímica, Málaga, Spain
Universidad de Málaga, SCAI, Servicio de Cultivo de Plantas, Málaga, Spain


  1. Palomo J, Gallardo F, Suárez MF, Cánovas C: Purification and characterization of NADP+-linked isocitrate dehydrogenase from Scots pine. Plant Physiol. 1998, 118: 617-626. 10.1104/pp.118.2.617.PubMed CentralView ArticlePubMedGoogle Scholar
  2. Pascual MB, Molina-Rueda JJ, Cánovas FM, Gallardo F: Spatial distribution of cytosolic NADP+-isocitrate dehydrogenase in pine embryos and seedlings. Tree Physiol. 2008, 28: 1773-1782.View ArticlePubMedGoogle Scholar
  3. Molina-Rueda JJ, Pascual MB, Cánovas FM, Gallardo F: Characterization and developmental expression of a glutamate decarboxylase from maritime pine. Planta. 2010, 232: 1471-1483. 10.1007/s00425-010-1268-9.View ArticlePubMedGoogle Scholar


© Molina-Rueda et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.