Efficient production of recombinant IgG by the GLUT5 co-expression system
© Inoue et al; licensee BioMed Central Ltd. 2011
Published: 22 November 2011
A fructose containing cell culture medium has the advantage of low lactate production and a small pH change, leading to cell and product stability. But, not all cell lines grow well in the medium, and the fructose transporter, GLUT5, is related to it . Thus, we developed an efficient production system of recombinant proteins by metabolic control and co-expression with GLUT5 in a fructose-based medium . In this report, the availability of the GLUT5 co-expression system was indicated in CHO-K1 and the human cell line, SC-01MFP .
Proliferation and IgG production in the fructose-based medium.
Relative cell proliferation
Relative IgG production
1.02 ± 0.19
1.77 ± 0.59
0.86 ± 0.01
1.84 ± 0.04
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