Volume 5 Supplement 8

22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies

Open Access

Efficient production of recombinant IgG by the GLUT5 co-expression system

BMC Proceedings20115(Suppl 8):P50

https://doi.org/10.1186/1753-6561-5-S8-P50

Published: 22 November 2011

A fructose containing cell culture medium has the advantage of low lactate production and a small pH change, leading to cell and product stability. But, not all cell lines grow well in the medium, and the fructose transporter, GLUT5, is related to it [1]. Thus, we developed an efficient production system of recombinant proteins by metabolic control and co-expression with GLUT5 in a fructose-based medium [2]. In this report, the availability of the GLUT5 co-expression system was indicated in CHO-K1 and the human cell line, SC-01MFP [3].

As a model, an IgG and GLUT5 co-expression vector was constructed and transfected into cells. When the transfected CHO-K1 and SC-01MFP cells were cultured in the fructose-based medium, both IgG productions were increased up to about two-fold of that cultured in the glucose-based medium (Table 1). Our study may be useful for efficient production of recombinant proteins using the fructose-based cell culture. In particular, the production in SC-01MFP cells is valuable for functional analysis of recombinant proteins with a human glycosylation profile.
Table 1

Proliferation and IgG production in the fructose-based medium.

Cell line

Relative cell proliferation

Relative IgG production

CHO-GLUT5/IgG

1.02 ± 0.19

1.77 ± 0.59

SC-01-GLUT5/IgG

0.86 ± 0.01

1.84 ± 0.04

Cells (1 x 105 cells/ml) were cultured in the glucose- and fructose-based media. After 3 days, cell proliferation and recombinant IgG production were compared between two media. Each value in the glucose-based culture is estimated as 1.00. Data represent relative values of means ± SD (n = 3).

Authors’ Affiliations

(1)
The Cell Engineering Center, Kitakyushu National College of Technology
(2)
KYURIN CORPORATION

References

  1. Inoue Y, Kawahara H, Shirahata S, Sugimoto Y: Retinoic acid improves a hybridoma culture in a fructose-based medium by up-regulation of fructose incorporation via retinoid nuclear receptors. Biosci Biotechnol Biochem. 2006, 70: 2248-2253. 10.1271/bbb.60178.View ArticlePubMedGoogle Scholar
  2. Inoue Y, Tsukamoto Y, Yamanaka M, Nakamura S, Inoue A, Nishino N, Kawahara H: Efficient production of recombinant IgG by metabolic control and co-expression with GLUT5 in a fructose-based medium. Cytotechnology. 2010, 62: 301-306. 10.1007/s10616-010-9289-6.PubMed CentralView ArticlePubMedGoogle Scholar
  3. Kawahara H: Human cell stains for protein production, provided by selecting strains with high intracellular protein and mutating with carcinogens. UK Patent. 2008, GB2426523Google Scholar

Copyright

© Inoue et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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