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Canine carcinomas in benign mixed tumours: versican expression and association with invasion


Components of the extracellular matrix have been studied in the attempt to elucidate the mechanisms involved with biological behavior of tumours. The presence of the proteoglycan versican has been strongly associated to cancer development and progression, however its relationship with invasion and tumoral progression has been little studied in veterinary medicine. Carcinomas in benign mixed tumours (CBMT) are one of the most common malignant tumour in female dogs and can serve as a model for studies on tumour progression. The aim of this paper was to evaluate the expression of versican in in situ and invasive carcinomatous areas of CBMT.

Materials and methods

Immunohistochemical staining for versican and confirmation of invasion areas with staining for p63 and smooth muscle α-actin (α-SMA) were performed on 49 cases of CBMT.


Invasion was considered when suspicious Haematoxylin-Eosin stained areas revealed a total loss of immunoreactivity for α-SMA and p63. Versican immunoreactivity was less intense adjacent to in situ carcinomatous regions when compared to invasive regions in which staining was found as more extensive areas with strong expression.


Obtained data reveal that, in carcinomas in benign mixed tumors, versican expression differs significantly within invasive and in situ areas, suggesting the role of this molecule in tumor progression.

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Correspondence to Geovanni D Cassali.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Damasceno, K.A., Bertagnolli, A.C., Estrela-Lima, A. et al. Canine carcinomas in benign mixed tumours: versican expression and association with invasion. BMC Proc 7 (Suppl 2), P39 (2013).

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