The role of genetic polymorphisms at the chromosomes 5p15, 6p12, 6p21 and 15q25 in non-small-cell lung cancer prognosis: a Portuguese prospective study
© de Mello et al; licensee BioMed Central Ltd. 2013
Published: 4 April 2013
Genome Wide Association Study (GWAS) variants on chromosome 15q25 and 5p15 and genetic polymorphisms on the vascular endothelial growth factor (VEGF) gene showed that they may contribute to lung carcinogenesis. Therefore, this study was performed in order to assess the role of GWAS, and VEGF variants in non-small-cell lung cancer (NSCLC) prognosis.
Materials and methods
Prospective study from February 2010 to April 2011. Median follow up was 12 months. NSCLC patient’s genotyping was performed using the Sequenom® MassARRAY platform. Kaplan-Meier curve was used to assess overall survival (OS) and progression-free-survival (PFS). Statistical significance was considered for p < 0.05.
144 NSCLC patients were consecutively genotyped in order to assess 19 single nucleotide polymorphisms (SNPs). Males were 78.5%. Median age was 61.5 (32 – 89) years-old. Non-squamous cell histology was 77.1% and 91.4% were stages IIIB and IV. The following SNPs showed influence in OS: rs2010963 (VEGF + 405 G/C), p = 0.042, rs3025010 (VEGF intron 5 C/T), p = 0.047, rs401681 C/T on 5p15, p = 0.046, rs31489 C/A on 5p15, p = 0.029; and these SNPs showed influence in PFS: rs9295740 G/A on 6p21, p = 0.074, rs401681 C/T on 5p15, p = 0.021.
This was the first large study in Portugal involving NSCLC patients and assessment of 19 SNPs on chromosome 5p15.33, 6p12, 6p21, 6p21.3, and 15q25. Our study suggests that variants on chromosome 5p15 and 6p21 are prognostic biomarkers in advanced NSCLC. In the future, genome-identified patients may improve NSCLC screening strategies and therapeutic management.
University of Minho, FAPESP and CAPES.
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