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  • Poster presentation
  • Open Access

Data integration methodology that couples novel bioreactor monitoring tools, automated sampling, and applied mathematics to redefine bioproduction processes

  • Lisa J Graham1Email author,
  • Jeffrey F Breit1,
  • Lynn A Davis1,
  • Corey C Dow-Hygeland1 and
  • Brandon J Downey1
BMC Proceedings20137(Suppl 6):P115

Published: 4 December 2013


Feedback SystemData IntegrationNutrient RequirementMonitoring ToolDevelopment Methodology

Cell physiology dynamically affects the nutrient requirements of a culture. It is critical to obtain data over appropriate time intervals to assess the impact of process conditions on the cell population. By optimizing bioreactor operation, feed strategies and media composition, we can limit the number of experiments to obtain the empirical data sets.

For this poster, we present an emerging process-development methodology that is based on applying novel and existing bioreactor monitoring technologies, coupled with applied mathematics, to bioreactor processes. This approach employs tools like dielectric spectroscopy, aseptic autosamplers, and cell-based bioreactor models. We will illustrate how information gained from these tools can be coupled through utilization of the proper data integration and applied mathematics techniques.

The knowledge gained using this improved process development methodology also supports a less-invasive monitoring and feedback system, and can be implemented using a customized bioreactor control code.

Authors’ Affiliations

Bend Research Inc, USA


© Graham et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.