Volume 7 Supplement 6

23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health

Meeting abstracts

Edited by Hansjörg Hauser

Publication charges for this supplement were funded by the European Society for Animal Cell Technology (ESACT). The Supplement Editor declares that he has no competing interests.

23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health.

Lille, France23-26 June 2013

Concatenated PDF 7S6

Page 1 of 3

A novel genotype of MVA that efficiently replicates in single cell suspensions

Authors: Ingo Jordan and Volker Sandig

Citation: BMC Proceedings 2013 7(Suppl 6):O1

Content type: Oral presentation Published on: 4 December 2013
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Electrically modulated attachment and detachment of animal cells cultured on an ITO patterning electrode surface

Authors: Sumihiro Koyama

Citation: BMC Proceedings 2013 7(Suppl 6):O2

Content type: Oral presentation Published on: 4 December 2013
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Novel strategy for a high-yielding mAb-producing CHO strain (overexpression of non-coding RNA enhanced proliferation and improved mAb yield)

Authors: Hisahiro Tabuchi

Citation: BMC Proceedings 2013 7(Suppl 6):O3

Content type: Oral presentation Published on: 4 December 2013
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Improvement in a human IgE-inducing system by in vitro immunization

Authors: Shuichi Hashizume and Hiroharu Kawahara

Citation: BMC Proceedings 2013 7(Suppl 6):O4

Content type: Oral presentation Published on: 4 December 2013
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First CpG island microarray for genome-wide analyses of DNA methylation in Chinese hamster ovary cells: new insights into the epigenetic answer to butyrate treatment

Authors: Anna Wippermann, Sandra Klausing, Oliver Rupp, Thomas Noll and Raimund Hoffrogge

Citation: BMC Proceedings 2013 7(Suppl 6):O5

Content type: Oral presentation Published on: 4 December 2013
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Aspects of vascularization in Multi-Organ-Chips

Authors: Katharina Schimek, Reyk Horland, Sven Brincker, Benjamin Groth, Ulrike Menzel, Ilka Wagner, Eva-Maria Materne, Gerd Lindner, Alexandra Lorenz, Silke Hoffmann, Mathias Busek, Frank Sonntag, Udo Klotzbach, Roland Lauster and Uwe Marx

Citation: BMC Proceedings 2013 7(Suppl 6):O6

Content type: Oral presentation Published on: 4 December 2013
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Construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering

Authors: Kyoung Ho Lee, Kohsuke Honda, Hisao Ohtake and Takeshi Omasa

Citation: BMC Proceedings 2013 7(Suppl 6):O7

Content type: Oral presentation Published on: 4 December 2013
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¹H-NMR spectroscopy for human 3D neural stem cell cultures metabolic profiling

Authors: Daniel Simão, Catarina Pinto, Ana P Teixeira, Paula M Alves and Catarina Brito

Citation: BMC Proceedings 2013 7(Suppl 6):O8

Content type: Oral presentation Published on: 4 December 2013
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BEAT^® the bispecific challenge: a novel and efficient platform for the expression of bispecific IgGs

Authors: Pierre Moretti, Darko Skegro, Romain Ollier, Paul Wassmann, Christel Aebischer, Thibault Laurent, Miriam Schmid-Printz, Roberto Giovannini, Stanislas Blein and Martin Bertschinger

Citation: BMC Proceedings 2013 7(Suppl 6):O9

Content type: Oral presentation Published on: 4 December 2013
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A quantitative and mechanistic model for monoclonal antibody glycosylation as a function of nutrient availability during cell culture

Authors: Ioscani Jiménez del Val, Antony Constantinou, Anne Dell, Stuart Haslam, Karen M Polizzi and Cleo Kontoravdi

Citation: BMC Proceedings 2013 7(Suppl 6):O10

Content type: Oral presentation Published on: 4 December 2013
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Generation of genetically engineered CHO cell lines to support the production of a difficult to express therapeutic protein

Authors: Holger Laux, Sandrine Romand, Anett Ritter, Mevion Oertli, Mara Fornaro, Thomas Jostock and Burkhard Wilms

Citation: BMC Proceedings 2013 7(Suppl 6):P1

Content type: Poster presentation Published on: 4 December 2013
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Expansion of mesenchymal adipose-tissue derived stem cells in a stirred single-use bioreactor under low-serum conditions

Authors: Carmen Schirmaier, Stephan C Kaiser, Valentin Jossen, Silke Brill, Frank Jüngerkes, Christian van den Bos, Dieter Eibl and Regine Eibl

Citation: BMC Proceedings 2013 7(Suppl 6):P2

Content type: Poster presentation Published on: 4 December 2013
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Evaluating the effect of chromosomal context on zinc finger nuclease efficiency

Authors: Scott Bahr, Laura Cortner, Sara Ladley and Trissa Borgschulte

Citation: BMC Proceedings 2013 7(Suppl 6):P3

Content type: Poster presentation Published on: 4 December 2013
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Insights into monitoring changes in the viable cell density and cell physiology using scanning, multi-frequency dielectric spectroscopy

Authors: John Carvell, Lisa Graham and Brandon Downey

Citation: BMC Proceedings 2013 7(Suppl 6):P4

Content type: Poster presentation Published on: 4 December 2013
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Multidimension cultivation analysis by standard and omics methods for optimization of therapeutics production

Authors: Julia Gettmann, Christina Timmermann, Jennifer Becker, Tobias Thüte, Oliver Rupp, Heino Büntemeyer, Anica Lohmeier, Alexander Goesmann and Thomas Noll

Citation: BMC Proceedings 2013 7(Suppl 6):P5

Content type: Poster presentation Published on: 4 December 2013
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Toward a serum-free, xeno-free culture system for optimal growth and expansion of hMSC suited to therapeutic applications

Authors: Mira Genser-Nir, Sharon Daniliuc, Marina Tevrovsky and David Fiorentini

Citation: BMC Proceedings 2013 7(Suppl 6):P6

Content type: Poster presentation Published on: 4 December 2013
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Highly efficient inoculum propagation in perfusion culture using WAVE Bioreactor™ systems

Authors: Christian Kaisermayer and Jianjun Yang

Citation: BMC Proceedings 2013 7(Suppl 6):P7

Content type: Poster presentation Published on: 4 December 2013
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Intact cell MALDI mass spectrometry biotyping for "at-line" monitoring of apoptosis progression in CHO cell cultures

Authors: Sebastian Schwamb, Bogdan Munteanu, Björn Meyer, Carsten Hopf, Mathias Hafner and Philipp Wiedemann

Citation: BMC Proceedings 2013 7(Suppl 6):P8

Content type: Poster presentation Published on: 4 December 2013
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Seed train optimization for suspension cell culture

Authors: Tanja Hernández Rodríguez, Ralf Pörtner and Björn Frahm

Citation: BMC Proceedings 2013 7(Suppl 6):P9

Content type: Poster presentation Published on: 4 December 2013
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In vitro safety assessment of nanosilver with improved cell culture systems

Authors: Alina Martirosyan, Madeleine Polet and Yves-Jacques Schneider

Citation: BMC Proceedings 2013 7(Suppl 6):P10

Content type: Poster presentation Published on: 4 December 2013
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Model-based design of the first steps of a seed train for cell culture processes

Authors: Simon Kern, Oscar B Platas, Martin Schaletzky, Volker Sandig, Björn Frahm and Ralf Pörtner

Citation: BMC Proceedings 2013 7(Suppl 6):P11

Content type: Poster presentation Published on: 4 December 2013
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Novel approaches to render stable producer cell lines viable for the commercial manufacturing of rAAV-based gene therapy vectors

Authors: Verena V Emmerling, Karlheinz Holzmann, Karin Lanz, Stefan Kochanek and Markus Hörer

Citation: BMC Proceedings 2013 7(Suppl 6):P12

Content type: Poster presentation Published on: 4 December 2013
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Benchmarking of commercially available CHO cell culture media for antibody production

Authors: David Reinhart, Christian Kaisermayer, Lukas Damjanovic and Renate Kunert

Citation: BMC Proceedings 2013 7(Suppl 6):P13

Content type: Poster presentation Published on: 4 December 2013
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Advanced off-gas measurement using proton transfer reaction mass spectrometry to predict cell culture parameters

Authors: Timo Schmidberger and Robert Huber

Citation: BMC Proceedings 2013 7(Suppl 6):P14

Content type: Poster presentation Published on: 4 December 2013
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New peptide-based and animal-free coatings for animal cell culture in bioreactors

Authors: Youlia Serikova, Aurélie Joly, Géraldine Nollevaux, Martin Bousmanne, Wafa Moussa, Jonathan Goffinet, Jean-Christophe Drugmand, Laurent Jeannin and Yves-Jacques Schneider

Citation: BMC Proceedings 2013 7(Suppl 6):P15

Content type: Poster presentation Published on: 4 December 2013
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An integrated synchronization approach for studying cell-cycle dependent processes of mammalian cells under physiological conditions

Authors: Oscar B Platas, Uwe Jandt, Volker Sandig, Ralf Pörtner and An-Ping Zeng

Citation: BMC Proceedings 2013 7(Suppl 6):P16

Content type: Poster presentation Published on: 4 December 2013
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Evaluation of process parameters in shake flasks for mammalian cell culture

Authors: Oscar B Platas, Volker Sandig, Ralf Pörtner and An-Ping Zeng

Citation: BMC Proceedings 2013 7(Suppl 6):P17

Content type: Poster presentation Published on: 4 December 2013
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Online glucose-lactate monitoring and control in cell culture and microbial fermentation bioprocesses

Authors: Henry Weichert and Mario Becker

Citation: BMC Proceedings 2013 7(Suppl 6):P18

Content type: Poster presentation Published on: 4 December 2013
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Study of the improved Sf9 transient gene expression process

Authors: Xiao Shen, David L Hacker, Lucia Baldi and Florian M Wurm

Citation: BMC Proceedings 2013 7(Suppl 6):P19

Content type: Poster presentation Published on: 4 December 2013
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Development of a Drosophila S2 insect-cell based placental malaria vaccine production process

Authors: Wian A de Jongh, Mafalda dos SM Resende, Carsten Leisted, Anette Strøbæk, Besim Berisha, Morten A Nielsen, Ali Salanti, Kathryn Hjerrild, Simon Draper and Charlotte Dyring

Citation: BMC Proceedings 2013 7(Suppl 6):P20

Content type: Poster presentation Published on: 4 December 2013
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Understanding the complexity of hydrolysates

Authors: Abhishek J Gupta, Kathleen Harrison and Dominick Maes

Citation: BMC Proceedings 2013 7(Suppl 6):P21

Content type: Poster presentation Published on: 4 December 2013
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Developing a production process for influenza VLPs: a comparison between HEK 293SF and Sf9 production platforms

Authors: Christine M Thompson, Emma Petiot, Marc G Aucoin, Olivier Henry and Amine A Kamen

Citation: BMC Proceedings 2013 7(Suppl 6):P22

Content type: Poster presentation Published on: 4 December 2013
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Dynamic cyclin profiles as a tool to segregate the cell cycle

Authors: David Garcia Munzer, Margaritis Kostoglou, Michalis C Georgiadis, Efstratios N Pistikopoulos and Athanasios Mantalaris

Citation: BMC Proceedings 2013 7(Suppl 6):P23

Content type: Poster presentation Published on: 4 December 2013
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Development and implementation of a global Roche cell culture platform for production of monoclonal antibodies

Authors: Thomas Tröbs, Sven Markert, Ulrike Caudill, Oliver Popp, Martin Gawlitzek, Masaru Shiratori, Chris Caffalette, Robert Shawley, Steve Meier, Abby Pynn, Wendy Hsu and Andy Lin

Citation: BMC Proceedings 2013 7(Suppl 6):P24

Content type: Poster presentation Published on: 4 December 2013
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Powerful expression in Chinese Hamster Ovary cells using bacterial artificial chromosomes: parameters influencing productivity

Authors: Wolfgang Sommeregger, Andreas Gili, Thomas Sterovsky, Emilio Casanova and Renate Kunert

Citation: BMC Proceedings 2013 7(Suppl 6):P25

Content type: Poster presentation Published on: 4 December 2013
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INVect - a novel polycationic reagent for transient transfection of mammalian cells

Authors: Sebastian Püngel, Miklos Veiczi, Tim Welsink, Daniel Faust, Vanessa Vater, Derek Levison, Uwe Möller and Wolfgang Weglöhner

Citation: BMC Proceedings 2013 7(Suppl 6):P26

Content type: Poster presentation Published on: 4 December 2013
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Development of a chemically defined cultivation and transfection medium for HEK cell lines

Authors: Sebastian Püngel, T Tim Welsink, Penélope Villegas Soto, Wolfgang Weglöhner, Tim F Beckmann, Ina Eickmeier, Stefan Northoff and Christoph Heinrich

Citation: BMC Proceedings 2013 7(Suppl 6):P27

Content type: Poster presentation Published on: 4 December 2013
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Automated substance testing for lab-on-chip devices

Authors: Lutz Kloke, Katharina Schimek, Sven Brincker, Alexandra Lorenz, Annika Jänicke, Christopher Drewell, Silke Hoffmann, Mathias Busek, Frank Sonntag, Norbert Danz, Christoph Polk, Florian Schmieder, Alexey Borchanikov, Viacheslav Artyushenko, Frank Baudisch, Mario Bürger…

Citation: BMC Proceedings 2013 7(Suppl 6):P28

Content type: Poster presentation Published on: 4 December 2013
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NIR-spectroscopy for bioprocess monitoring & control

Authors: Marko Sandor, Ferdinand Rüdinger, Dörte Solle, Roland Bienert, Christian Grimm, Sven Groß and Thomas Scheper

Citation: BMC Proceedings 2013 7(Suppl 6):P29

Content type: Poster presentation Published on: 4 December 2013
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Case study: biosimilar anti TNFalpha (Adalimumab) analysis of Fc effector functions

Authors: Carsten Lindemann, Silke Mayer, Miriam Engel and Petra Schroeder

Citation: BMC Proceedings 2013 7(Suppl 6):P30

Content type: Poster presentation Published on: 4 December 2013
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Cellular tools for biosimilar mAb analysis

Authors: Carsten Lindemann, Silke Mayer, Miriam Engel and Petra Schroeder

Citation: BMC Proceedings 2013 7(Suppl 6):P31

Content type: Poster presentation Published on: 4 December 2013
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The successful transfer of a modern CHO fed-batch process to different single-use bioreactors

Authors: Sebastian Ruhl, Ute Husemann, Elke Jurkiewicz, Thomas Dreher and Gerhard Greller

Citation: BMC Proceedings 2013 7(Suppl 6):P32

Content type: Poster presentation Published on: 4 December 2013
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Differences in the production of hyperglycosylated IFN alpha in CHO and HEK 293 cells

Authors: Agustina Gugliotta, Marcos Oggero-Eberhardt, Marina Etcheverrigaray, Ricardo Kratje and Natalia Ceaglio

Citation: BMC Proceedings 2013 7(Suppl 6):P33

Content type: Poster presentation Published on: 4 December 2013
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Developing an upstream process for a monoclonal antibody including medium optimization

Authors: Sevim Duvar, Volker Hecht, Juliane Finger, Matthias Gullans and Holger Ziehr

Citation: BMC Proceedings 2013 7(Suppl 6):P34

Content type: Poster presentation Published on: 4 December 2013
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Development and evaluation of a new, specially tailored CHO media platform

Authors: Tim F Beckmann, Christoph Heinrich, Heino Büntemeyer and Stefan Northoff

Citation: BMC Proceedings 2013 7(Suppl 6):P35

Content type: Poster presentation Published on: 4 December 2013
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Streamlined process development using the Micro24 Bioreactor system

Authors: Steve RC Warr, John PJ Betts, Shahina Ahmad, Katy V Newell and Gary B Finka

Citation: BMC Proceedings 2013 7(Suppl 6):P36

Content type: Poster presentation Published on: 4 December 2013
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Temperature dependency of immunoglobulin production in novel human partner cell line

Authors: Galina Kaseko, Marjorie Liu, Edwin Hoe, Qiong Li, Mercedes Ballesteros and Tohsak Mahaworasilpa

Citation: BMC Proceedings 2013 7(Suppl 6):P37

Content type: Poster presentation Published on: 4 December 2013
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Strategies for clone detection, selection and isolation in Per.C6 cells - case for Rebmab100

Authors: Fernanda P Yeda, Mariana L dos Santos, Lilian R Tsuruta, Bruno B Horta, André L Inocencio, Oswaldo K Okamoto, Maria C Tuma and Ana M Moro

Citation: BMC Proceedings 2013 7(Suppl 6):P38

Content type: Poster presentation Published on: 4 December 2013
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Impact of single-use technology on continuous bioprocessing

Authors: William G Whitford and Brandon L Pence

Citation: BMC Proceedings 2013 7(Suppl 6):P39

Content type: Poster presentation Published on: 4 December 2013
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Comparison of BHK-21 cell growth on microcarriers vs in suspension at 2L scale both in conventional bioreactor and single-use bioreactor (Univessel^® SU)

Authors: Lídia Garcia, Elisenda Viaplana and Alicia Urniza

Citation: BMC Proceedings 2013 7(Suppl 6):P40

Content type: Poster presentation Published on: 4 December 2013
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23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health

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