Understanding the complexity of hydrolysates
© Gupta et al.; licensee BioMed Central Ltd. 2013
Published: 4 December 2013
Hydrolysates are complex media supplements composed of many as well as different types of compounds. Within Frieslandcampina Domo's Quality by Design project, detailed information of these compounds (annotation and quantification) has been generated. This was achieved for soy protein hydrolysates (Proyield Soy SE50MAF-UF) using metabolomics biochemical profiling. Biochemical profiling, together with peptide profiling and analysis of the inorganic compounds, resulted in complete characterization of this hydrolysate product. Additionally, these lots of Proyield Soy SE50MAF-UF were tested for cell culture performance.
Results and Discussion
The composition data was natural log transformed and functionality data was corrected for experiment-to-experiment variation. Consequently, the dataset was analyzed using statistical tools like two-mode cluster analysis, bootstrapped stepwise regression and 2D correlation analysis. These statistical tools were composed in-house using Matlab® R 2009b version 188.8.131.529.
Key compounds supplemented at 0.01% (w/v) to CD media.
Specific IgG production (%)*
This suggests that the effect of a hydrolysate cannot by mimicked by adding certain key compounds. Alternatively, this suggests that these key compounds are biomarkers, which are interconnected with several other compounds, and that presence of all of these compounds is relevant/important for the enhancement in the functionality.
In hydrolysates, these compounds interact with several other compounds in a complex biochemical network. This network of compounds is a unique and native feature of hydrolysates and non-existent in chemically defined media.
Working in close collaboration with our customers, we gain understanding about the relation between the complex composition of hydrolysates and their effect on cell growth and titer in the application.
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