Novel strategy for a high-yielding mAb-producing CHO strain (overexpression of non-coding RNA enhanced proliferation and improved mAb yield)
© Tabuchi; licensee BioMed Central Ltd. 2013
Published: 4 December 2013
Innovation in mAb production is driven by strategies to increase yield. A host cell line constructed to overexpress TAUT (taurine transporter) produced a higher proportion of high-mAb-titer strains . From these we selected a single TAUT/mAb strain that remained viable for as long as 1 month. Its improved viability is attributed to improved metabolic properties. It was also more productive (>100 pg/cell/day) and yielded more mAb (up to 8.1 g/L/31 days) than the parent cell line . These results suggested that this host cell engineering strategy has great potential for the improvement of mAb-producing CHO cells.
1.We found a lncRNA that was abnormally expressed in high-titer cells. It was identified as the antisense RNA of NFKBIA. Overexpression of part of the lncRNA suppressed NFKBIA mRNA.
2. Overexpression of part of the lncRNA improved CHO cell performance. The transporter/lncRNA co-overexpressing strain gave increased yield in a shorter culture period.
3. This effect might not be due to enhancement of the NFKB-dependent promoter of the mAb expression plasmid.
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