Volume 7 Supplement 6
Improvement in a human IgE-inducing system by in vitro immunization
© Hashizume and Kawahara; licensee BioMed Central Ltd. 2013
Published: 4 December 2013
The immune system, which is the self-defense system of the body, occasionally responds in a manner that is harmful to the body. The incidence and severity of allergies caused by cedar pollen, house dust, egg protein, and many others are increasing and have recently become a serious social problem. We have previously developed an original in vitro system for inducing human IgE antibody specific to a designated antigen that can be used to study various allergic reaction . In this study, we attempted to improve this system to stimulate IgE levels in its medium to provide a highly sensitive screening method.
The original in vitro IgE-inducing system was established using lymphocytes and plasma from donors which were not naturally immunized with allergens. The original system contained ERDF supplemented with fetal bovine serum (final concentration, 5%) and contained human plasma (10%) as an essential component. Human peripheral blood lymphocytes and plasma were obtained by density-gradient centrifugation at 400 × g for 30 min with cell separation medium, Ficoll-Paque™ Plus. This system also included allergen (100 ng/ml), interleukins (IL-) 2, 4, and 6 (10 ng/ml each) and muramyl dipeptide (MDP, 10 μg/ml), as described previously . Human lymphocytes were cultured in 96- or 24-well plates at a final density of 1 × 106 cells/ml in the medium and incubated in a CO2 incubator at 37°C for 10 days. During the 10 days, IgE was specifically secreted into the medium.
Results and discussion
Effects of human plasma and interleukins on human IgE induction
The necessity for inclusions of human plasma and interleukins was shown, when human lymphocytes and plasma from donors which were not naturally immunized with allergens were used. For the induction of IgE, human lymphocytes and plasma obtained from the same donor were required . Addition of IL-2, 4 and 6 induced IgE. Elimination of each of these three interleukins from the medium resulted in no induction of IgE (data not shown). From these results, IL-2, 4 and 6 are considered to be essential factors to initially immunize lymphocytes with allergens, when lymphocytes and plasma from donors not naturally immunized with allergens were used. We next attempted to improve this system to stimulate IgE levels in the medium to provide a highly sensitive screening method.
Effects of elimination of IL-2 from the medium on human IgE production
Effects of various additives on IgE productivity
IgE productivity (ng/ml)
Control (ERDF + hPlasma + FBS)
319 ± 19
+ IL-2 + IL-4 + IL-6 + MDP + Cryj1
356 ± 85
+ IL-4 + IL-6 + MDP + Cryj1
549 ± 189
+ IL-4 + IL-6 + MDP + Cryj1 + strawberry extract
341 ± 55
+ IL-4 + IL-6 + MDP + Derf2
660 ± 172
+ IL-4 + IL-6 + MDP + Derf2 + strawberry extract
499 ± 167
In summary, elimination of IL-2 from the IgE-inducing system medium increased the IgE induction level when human lymphocytes and plasma obtained from donors naturally immunized with allergens were used. The level of about 1 μg/ml IgE reported to be secreted in this study may be the highest compared with those reported elsewhere. The original and improved systems for human IgE production are considered to be of profound use for studying allergy mechanisms and surveying allergy-alleviating products, respectively.
- Kawahara H, Maeda-Yamamoto M, Hakamata K: Effective induction and acquisition of human IgE antibodies reactive with house-dust mite extracts. J Immunol Methods. 2000, 233: 33-40.View ArticlePubMedGoogle Scholar
- Hashizume S, Kawahara H: Inducing of human IgE antibodies by in vitro immunization. Proceedings of the 20th Annual Meeting of the European Society for Animal Cell Technology (ESACT). Edited by: Noll T. 2010, Springer Science+Business Media B.V, 833-836. Dresden, Germany, 2007Google Scholar
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