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Marine natural compounds can be efficient toward aflatoxigenic Aspergillus flavus strain

  • Yasmim Dantas Crivelenti1,
  • Cyntia AM Arevabini1,
  • Mariana H De Abreu1,
  • Tamires Aparecida Bitencourt1,
  • Thaís B Mesquita1,
  • Bruna AM Cantelli1,
  • Mario FC Santos2,
  • Roberto GS Berlinck2,
  • Eduardo Hajdu3,
  • Renê de O Beleboni1,
  • Mozart Marins1 and
  • Ana Lúcia Fachin1
BMC Proceedings20148(Suppl 4):P114

Published: 1 October 2014


Minimum Inhibitory ConcentrationAntifungal AgentATCC StrainPromising SourceAflatoxin Produce


The presence of high levels of aflatoxin is a serious problem to the production of raw peanuts and peanut crumbs. The high incidence of aflatoxin in peanuts in our country is mainly due to problems in primary production. High humidity and temperature conditions increase the likelihood of Aspergillus development and aflatoxins production, which is worsened during rainy weather [1]. Aflatoxins may remain in the food after the death of fungus without visible alterations [2]. The effects of aflatoxins on human and animal health, besides resistance of fungi to conventional antifungal agents has motived the search for new inhibitors. The research of marine natural products from sponges has been considered as a promising source for the development of new antifungal agents in order to discover compounds more effective and less toxic [3]. The objective of this study was to evaluate the antifungal activity of 21 marine natural compounds toward an aflatoxigenic A.flavus ATCC strains.


Aflatoxin producing (CCT 7836) and non producing (ATCC9643) A. flavus strains were purchased from Fundação André Tosello, Campinas, Brazil and were kept in Sabouraud media at 35 ºC. The minimum inhibitory concentration (MIC) of 21 sponge marine natural products, identified as SM1 to SM21, toward the two strains of A.flavus was determined by microdilution assay in 96-well plates using RPMI medium according to the protocol NCCLS M-38 [4] for 7 days at 37C, using commercial antifungal cercobin as control.

Results and conclusions

The marine product SM5 (CIM = 3.9 µg/mL) was more effective than cercobin (CIM = 7.8 µg/mL) against A. flavus control aflatoxin producer strain. Moreover, marine product SM5 (MIC = 1.9 µg/mL) was also a more effective antifungal agent than cercobin (MIC = 3.9 µg/mL) against the A. flavus ATCC strain. The findings suggest that natural marine products are a promising source of new molecules for the development of antifungal compounds against aflatoxigenic fungus that affect public health and the food production.



This study was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo, CNPq, CAPES and PET-MEC.

Authors’ Affiliations

Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, Brazil
Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, Brazil
Museu Nacional, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil


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© Crivelenti et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.