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BMC Proceedings

Open Access

Purification, characterization and antifungal activity of LPA a lectin from Pachira aquatica seeds: their possible role in plant defence

  • Ana Luiza Paiva1,
  • Ilka Maria Vasconcelos1 and
  • José Tadeu Abreu Oliveira1
BMC Proceedings20148(Suppl 4):P134

https://doi.org/10.1186/1753-6561-8-S4-P134

Published: 1 October 2014

Background

The lectins are an important class of proteins ubiquitous in nature, which plays different roles and functions in biological processes such as recognition molecules within the immune system in animals and as storage proteins or in defence mechanisms against pest and pathogens in plants. Oliveira et al. [1] showed that rats fed on a lectin-rich diet from Pachira aquatica seeds had a poor growth performance and some internal organs affected. This work reports on the purification and characterization of a lectin from Pachira aquatica seeds, with the intention of elucidating its role in plant defence against fungal pathogens.

Methods

The crude extract of P. aquatica seeds was fractionated by precipitation with ammonium sulfate (0-60% saturation) and applied on an Agarose-N-acetyl-D-galactosamine affinity chromatography matrix. The purified lectin, named LPA, was incubated with three different fungal species to access its effect on spore germination and growth inhibition. Moreover, the resistance of LPA to proteolytic enzymes, a prerequisite of its defense role, was examined.

Results and conclusion

SDS-PAGE of the matrix-desorbed protein showed a single band with an apparent MW of 65 kDa. The purified lectin (LPA) inhibited the spore germination of Fusarium solani and Fusarium oxysporum and the vegetative growth of Colletotrichum gloeosporioides, F. solani and F. oxysporum. LPA also showed to be resistant to proteolysis invitro. These results suggest that LPA might contribute to the plant defense against pathogens and constitutes a candidate to improve plant resistance to some phytopathogenic fungi.

Declarations

Acknowledgements

This work was carried out in the department of Biochemistry and Molecular Biology in Federal University of Ceara and was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Programa de Apoio a Núcleos de Excelência (PRONEX), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Programa acional de Cooperação Acadêmica (PROCAD) and Fundação de Amparo a Pesquisa do Estado do Ceara (FUNCAP).

Authors’ Affiliations

(1)
Federal University of Ceara

References

  1. Oliveira JTA, Vasconcelos IM, Bezerra LCNM, Silveira SB, Monteiro ACO, Moreira RA: Composition and nutritional properties of seeds from Pachira aquatica Aubl,Sterculia striata St Hil et Naud and Terminalia catappa Linn. Food Chem. 2000, 70: 185-1.View ArticleGoogle Scholar

Copyright

© Paiva et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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