Purification, characterization and antifungal activity of LPA a lectin from Pachira aquatica seeds: their possible role in plant defence
© Paiva et al.; licensee BioMed Central Ltd. 2014
Published: 1 October 2014
The lectins are an important class of proteins ubiquitous in nature, which plays different roles and functions in biological processes such as recognition molecules within the immune system in animals and as storage proteins or in defence mechanisms against pest and pathogens in plants. Oliveira et al.  showed that rats fed on a lectin-rich diet from Pachira aquatica seeds had a poor growth performance and some internal organs affected. This work reports on the purification and characterization of a lectin from Pachira aquatica seeds, with the intention of elucidating its role in plant defence against fungal pathogens.
The crude extract of P. aquatica seeds was fractionated by precipitation with ammonium sulfate (0-60% saturation) and applied on an Agarose-N-acetyl-D-galactosamine affinity chromatography matrix. The purified lectin, named LPA, was incubated with three different fungal species to access its effect on spore germination and growth inhibition. Moreover, the resistance of LPA to proteolytic enzymes, a prerequisite of its defense role, was examined.
Results and conclusion
SDS-PAGE of the matrix-desorbed protein showed a single band with an apparent MW of 65 kDa. The purified lectin (LPA) inhibited the spore germination of Fusarium solani and Fusarium oxysporum and the vegetative growth of Colletotrichum gloeosporioides, F. solani and F. oxysporum. LPA also showed to be resistant to proteolysis invitro. These results suggest that LPA might contribute to the plant defense against pathogens and constitutes a candidate to improve plant resistance to some phytopathogenic fungi.
This work was carried out in the department of Biochemistry and Molecular Biology in Federal University of Ceara and was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Programa de Apoio a Núcleos de Excelência (PRONEX), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Programa acional de Cooperação Acadêmica (PROCAD) and Fundação de Amparo a Pesquisa do Estado do Ceara (FUNCAP).
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