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BMC Proceedings

Open Access

Preliminary analysis of the transcriptome of salivary glands of Ornithodoros brasiliensis (Acari: Argasidae)

  • DNS Giovanni1,
  • GA Landulfo2,
  • LL Duarte2,
  • FF Santos2,
  • PL Ho3,
  • RZ Mendonça1,
  • E Carvalho3,
  • ILM Junqueira-de-Azevedo4,
  • LAB Proença4 and
  • DM Barros-Battesti2
BMC Proceedings20148(Suppl 4):P145

https://doi.org/10.1186/1753-6561-8-S4-P145

Published: 1 October 2014

Ornithodoros brasiliensis Aragão is an endemic tick to Brazil, restricted to highlands of the state of Rio Grande do Sul[1]. It is a very aggressive species to humans, causing fever, great pain and intense inflammatory response at the bite site[2]. To build a cDNA library from the salivary glands of this tick. Pirosequencing was used, Model GS-454 Junior to sequencing the cDNA obtained from mRNA from the salivary glands, producing a total of 81,178 sequences (reads) with an average size of 400.8 bases. Were assembled 4,558 contigs using the program Genomics CLC-(CLCBio) and annotated by software Blast2GO, based on sequence similarity. These were compared to various banks (GenBank, GO, Enzyme Codes, InterPro, KEGG). The results showed that 20.93% of them were classified as belonging to the secretory pathway and 14.77% as proteins of membrane, confirming the tissue of salivary gland. The main families of transcribed genes were lipocalins (15.6%), moubatins which can be sub-classes of lipocalins (6.23%), protein with a tail acid (1.63%), and metalloproteases (1.23%). The sequences of interest present in the salivary glands were cloned into bacterial heterologous system[35]. The recombinant proteins were expressed in sufficient quantities to analyze the sequences of lipocalins, moubatins, metalloproteases, cell cycle modulating and related to analgesia. All these proteins show functions that modify the coagulation activities, modulating the immune response and inhibition of platelet aggregation, among others, showing their potential for development of bioproducts with medical and industrial interests.

Authors’ Affiliations

(1)
Laboratório de Parasitologia, Instituto Butantan
(2)
Laboratório Especial de Coleções Zoológicas, Instituto Butantan
(3)
Laboratório de Biotecnologia, Instituto Butantan
(4)
Laboratório Especial de Toxinologia Aplicada, Instituto Butantan

References

  1. Aragão HB: Ornithodoros brasiliensis. Brazil Médico. 1923, 37: 20-Google Scholar
  2. Davis GE: Observations on the biology of the argasid tick, Ornithodoros brasiliensis Aragao, 1923; with the recovery of a spirochete, Borrelia brasiliensis, n. sp. J Parasitol. 1952, 38: 473-476. 10.2307/3273927.View ArticlePubMedGoogle Scholar
  3. Mans BJ, Andersen JF, Francischetti IM, Valenzuela JG, Schwan TG, Pham VM, Garfield MK, Hammer CH, Ribeiro JM: Comparative sialomics between hard and soft ticks: implications for the evolution of blood-feeding behavior. Insect Biochem Mol Biol. 2008, 38: 42-58. 10.1016/j.ibmb.2007.09.003.PubMed CentralView ArticlePubMedGoogle Scholar
  4. Francischetti IM, Mather TN, Ribeiro JM: Cloning of a salivary gland metalloprotease and characterization of gelatinase and fibrin(ogen)lytic activities in the saliva of the Lyme disease tick vector Ixodes scapularis. Biochem Biophys Res Commun. 2003, 305: 869-875. 10.1016/S0006-291X(03)00857-X.PubMed CentralView ArticlePubMedGoogle Scholar
  5. Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E: A novel family of anticoagulants from the saliva of Ixodes scapularis. Insect Mol Biol. 2002, 11: 641-650. 10.1046/j.1365-2583.2002.00375.x.View ArticlePubMedGoogle Scholar

Copyright

© Giovanni et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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