Use of phytopathogenic virus for peptide expression in plant system
© de Sousa et al.; licensee BioMed Central Ltd. 2014
Published: 1 October 2014
Some plant viruses have been used to express and / or car peptides from human and animal pathogens for diagnostic purposes or vaccine. This system has presented many advantages over traditional methods, especially with regard to cost and production of peptides free of pathogens . In this context, the possibility of using these viruses in an effort to produce peptide vaccine candidates in veterinary medicine is presented as a very promising idea. Among the relevant diseases in veterinary medicine there iscaprine artritre encephalitis (CAE) virus infection caprine arthritis-encephalitis (CAEV). This virus infects goats worldwide causing arthritis, encephalitis, mastitis, progressive weight loss and mainly fall in production and consequently a major economic loss to producers . This disease is silent and yet there is still no treatment nor vaccine, and the control accomplished by early diagnosis. Therefore, this study aimed to produce peptides CAEV using a plant system.
Primers were designed for a segment of p28 protein from the gag gene of CAEV. The insert obtained was cloned into pGEM, inserted into plasmid non-commercial (PNC) and inoculated on susceptible plants. The plants were kept in a greenhouse until the appearance of symptoms. The extraction of proteins was carried out  and the sample was submitted to SDS-PAGE and Western Blotting assay.
Results and conclusions
The electrophoretic profile of approximately 55 kDa for the produced protein was similar to the positive control (commercial p28 protein). The Western Blotting confirmed the specific reactivity to this antigen. Thus, it was possible to determine the production of CAEV vaccine candidate peptides in plant systems.
The authors are thankful to the Rede Nordeste de Biotecnologia (RENORBIO).
- Porta C, Lomonossoff GP: Scope for using plant viruses to present epitopes from animal pathogens. Rev Med Virol. 1998, 8: 25-41. 10.1002/(SICI)1099-1654(199801/03)8:1<25::AID-RMV212>3.0.CO;2-V.View ArticlePubMedGoogle Scholar
- Smith MC, Sherman DM: Goat medicine. Philadelphia, Lea & Febiger. 1994Google Scholar
- Florindo MIG, Aragão MEF, Melo DF, Otoch MLO, Lima MGS: Immune response induced in mice by oral immunization with cowpea severe mosaic virus. Braz J Med Biol Res. 2002, 35: 827-835.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.