Evaluation of antimicrobial potential of leaf extract of plants collected in the UFAM campus' reserve
© Carvalho et al.; licensee BioMed Central Ltd. 2014
Published: 1 October 2014
The search for substances with antimicrobial properties is intensifying every day, given the increasing number of microorganisms resistant to the usual antibiotics. The use of plant components in the pharmaceutical field has gradually increased in Brazil. According to the World Health Organization, medicinal plants should be the best sources to obtain up new varieties of drugs . Due to the above, there was the need to assess the antimicrobial potential in some species of Myrtaceae, Anacardiaceae and Oxalidaceae families.
The leaves were collected in domestic and native plants in the reserve of the university campus. The collected material was triturated in distilled water, centrifuged 8000xg and the supernatant filtered through 0.22-µm membrane and stored in 15mL-Falcon tube . The leaves were dried at 50 °C for 24h. The antimicrobial activity tests were the agar diffusion (Kirby-Bauer), and when confirmed the presence of antagonism one bioautography was performed to determine the Rf of antibiotic molecules. Test microorganisms were spiked in Plate Count Agar medium (PCA) and incubated at 37°C for 24 hours before testing . For inoculum preparation, the youth cultures of each microorganism were standardized in sterile saline according to the 0.5-MacFarland Scale. The tested extracts included the filtrate liquid and the hydrated powder 10% (w/v). The seeding was carpet-type using "Swab", 100µL of extracts were placed in "Cup plate" incubated for 24 h at 37 °C. The positive extracts in the biological assays were weighed and serially diluted to twice with distilled water (200 mg / 2 mL) and transferring one mL of this dilution to the subsequent tube to 10-8 in In each tube containing the respective dilution by adding 19.0 mL of Mueller-Hinton agar, which was poured into Petri dishes. Thus, the concentration range from 5 to 0.04 mg / mL was obtained. The seeding will be carpet type using "Swab". The MIC will be considered the lowest concentration that inhibited microbial growth .
Results and discussion
Species of Myrtaceae, Anacardiaceae and Oxalidaceae famiies showed positive results against the test microorganisms Candida albicans, Escherichia coli, Mycobacterium smegmatis and Staphylococcus aureus. In the study concerning to the minimum inhibitory concentration (MIC), it was notorious that better results were obtained when using more concentrated extract. The mean inhibitory concentration for samples was 10-².The results show that the species of the three families has great antimicrobial potential. Noting the ability of Amazon to research and production of new drugs.
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