- Poster presentation
- Open access
- Published:
Evidence of a new intermediate compound of the chitin biogenesis found in the marine-derived Penicilliumroqueforti fungus
BMC Proceedings volume 8, Article number: P212 (2014)
Chitin derivatives, chitosan and substituted chito-oligosaccharides from fungi present a wide spectrum of applications and they have been studied in many fields such as medicine, cosmetics, agriculture, aquaculture, and food as dietary supplements [1]. Chitin is a copolymer with N-acetyl-D-glucosamine units linked with β-(1-4)-glycosidic bonds which provide rigidity to the cell wall in chitinous fungi. There is evidence that the chitin synthesis is catalyzed by the chitin synthase (CS; EC 2.4.1.16), an enzyme that transfers β-1,4-linked anhydro-2-acetamido-2-deoxy-D-glucose (GlcNAc) from uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) to the nonreducing end of growing chitin chains [2]. However, chitin is synthesized by the regulation of distinct isoenzymes whose number ranges in some hyphomycetes [3]. Nevertheless, there is relatively little information on the genes responsible for chitin biosynthesis in filamentous fungi, analyses of DNA fragments from taxonomically diverse fungal species have shown that most fungi have three to six chitin synthase genes [4]. Therefore, this diversity of chitin synthases makes difficult to find a unique model of regulation of the chitin pathway. The chemical screening of the biomass of a new marine-derived strain of Penicilliumroqueforti, produced by liquid-state fermentation, led to the identification of several volatile and non-volatile compounds [5]. As a result of this previous study, we have isolated and characterized a new molecule. The chemical structure of the 2-deoxy-2-phosphamino-α-D-glucopyranose isolated was elucidated on the basis of 1D and 2D NMR studies as well as other instrumental techniques. In consequence of this discovery, it has been proposed a biogenetic route aiming to explain its formation as an intermediary component of the chitin biosynthesis.
References
Jayakumar R, Menon D, Manzoor K, Nair SV, Tamura H: Biomedical applications of chitin and chitosan based nanomaterials - A short review. Carbohyd Polym. 2010, 82 (2): 227-232. 10.1016/j.carbpol.2010.04.074.
Bulik DA, Olczak M, Lucero HA, Osmond BC, Robbins PW, Specht CA: Chitin synthesis in Saccharomyces cerevisiae in response to supplementation of growth medium with glucosamine and cell wall stress. Eukaryot Cell. 2003, 29 (5): 886-900.
Hartl L, Zach S, Seidl-Seiboth V: Fungal chitinases: diversity, mechanistic properties and biotechnological potential. Appl Microbiol Biot. 2012, 93 (2): 533-43. 10.1007/s00253-011-3723-3.
Gandía M, Harries E, Marcos JF: Identification and characterization of chitin synthase genes in the postharvest citrus fruit pathogen Penicillium digitatum. Fungal Biol. 2012, 116 (6): 654-664. 10.1016/j.funbio.2012.03.005.
Mioso R, Toledo Marante FJ, Bravo de Laguna IH, González JEG, Rodríguez JJS: Biomolecules produced in liquid-state fermentation by a marine-derived fungus, Penicillium roqueforti. Química Nova. accepted
Author information
Authors and Affiliations
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Marante, F.J.T., Mioso, R., Laguna, I.H.B.d. et al. Evidence of a new intermediate compound of the chitin biogenesis found in the marine-derived Penicilliumroqueforti fungus. BMC Proc 8 (Suppl 4), P212 (2014). https://doi.org/10.1186/1753-6561-8-S4-P212
Published:
DOI: https://doi.org/10.1186/1753-6561-8-S4-P212