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BMC Proceedings

Open Access

Antimicrobial activity of the ethanolic extract of Guettarda sericeaagainst oral streptococci

  • Edson Teixeira1,
  • Érica Rabelo1,
  • Bruno Silva1,
  • Luiz Nascimento Neto1,
  • Francisco Arruda1,
  • Hélcio Santos2,
  • Maria Albuquerque2,
  • Paulo Bandeira2,
  • Kyria Nascimento1,
  • Benildo Cavada1 and
  • Jorge Martins3
BMC Proceedings20148(Suppl 4):P54

https://doi.org/10.1186/1753-6561-8-S4-P54

Published: 1 October 2014

Background

The genus Guettarda (Rubiaceae) comprises plants widely distributed in tropical areas [1]. Regarding the Guettarda sericea species, the literature shows that there is a lack of botanical and phytochemicals studies [2]. Thus, the present study aimed to evaluate the antibacterial effect of ethanol extract of leaves of G. sericea (EEFGS) on the growth of Streptococcus oralis ATCC 10557 and S. salivarius ATCC 7073 in both the planktonic and biofilms states.

Methods

Different methods were employed to verify the antimicrobial potential. Among these are the determination of minimum inhibitory concentration (MIC) determination of the death curve and evaluation of minimum bactericidal concentration (MBC) [3]. Furthermore, quantification of biomass and the number of viable cells of the biofilm were performed [4]. The negative and positive controls used in all assays were respectively 4% DMSO and chlorhexidine gluconate. To determine the toxicity of EEFGS, it was used the toxicity test on Artemia nauplii [5].

Results and conclusions

The data showed that the extract has a remarkable antimicrobial effect, able to inhibit the growth of planktonic and development of biofilms of S. oralis strain until the concentration of 62.5 μg.mL-1. Regarding the toxicity, it was observed that death of Artemia nauplii occurred at higher concentrations than those that exhibited antibacterial effect. It can be concluded that EEFGS can be used as agents for the control of biofilm formation of S. oralis. In addition, complementary methodologies that seek purification of the active compounds and their cytotoxic effects on eukaryotic cells need to be held, aiming its use as an herbal agent.

Authors’ Affiliations

(1)
Federal University of Ceará
(2)
Acaraú Valley State University
(3)
Federal University of Pelotas

References

  1. Achille F, Motley TJ, Lowry II PJ, Jérémie J: Polyphyly in Guettarda L. (Rubiaceae, Guettardeae) based on nrDNA ITS sequence data. Annals of the Missouri Botanical Garden. 2006, 93 (1): 103-121. 10.3417/0026-6493(2006)93[103:PIGLRG]2.0.CO;2.View ArticleGoogle Scholar
  2. Agra MF, França PF, Barbosa-Filho JM: Synopsis of the plants known as medicinal and poisonous in Northeast of Brazil. Revista Brasileira de Farmacognosia. 2007, 17 (1): 114-140. 10.1590/S0102-695X2007000100021.View ArticleGoogle Scholar
  3. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing, twenty informational supplement, approved standard MS100-S20. 2010, Wayne, PA: CLSIGoogle Scholar
  4. Stepanovic S, Vukovic D, Dakic I, Savic B, Svabic-Vlahovic M: A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods. 2000, 40 (2): 175-9. 10.1016/S0167-7012(00)00122-6.View ArticlePubMedGoogle Scholar
  5. Veiga LF, Vital N: Testes de toxicidade aguda com o microcrustáceo Artemia sp. Métodos em Ecotoxicologia Marinha. Aplicações no Brasil. Edited by: Nascimento IA, Sousa ECPM, Nipper M. 2002, Artes Gráficas e Indústria Ltda: São PauloGoogle Scholar

Copyright

© Teixeira et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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