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BMC Proceedings

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Antimicrobial activity of the trans-cinnamaldehyde on nosocomial enteric bacilli producers of extended spectrum β-lactamase (ESBL)

  • Vicente Pinto1,
  • César Barbosa2,
  • Pedro Magalhães3,
  • Camila Coelho4,
  • Joseires Fontenelle5,
  • Gerardo Cristino-Filho1,
  • Helliada Chaves2,
  • Antonio Silva2,
  • Alrieta Teixeira2 and
  • Mirna Bezerra1
BMC Proceedings20148(Suppl 4):P89

Published: 1 October 2014


Antimicrobial ActivityMinimum Inhibitory ConcentrationCephalosporinNosocomial InfectionAztreonam


The extended spectrum ß-lactamases (ESBL) are enzymes that produce resistance to ß-lactam antibiotics, including penicillins, wide spectrum cephalosporins and aztreonam, by cleavage of ß-lactam ring (BUSH & JACOBY, 2010). Since ESBL-producing bacteria are frequently associated with nosocomial infections, treatment options are becoming increasingly limited (RAWAT & NAIR, 2010). In this context, the discovery of compounds which can inhibit the growth of micro-organisms which produce these enzymes becomes increasingly important.


In this study were evaluated the antimicrobial activity of trans-cinnamaldehyde by microdilution technique and also determined its minimum bactericidal concentration (MBC) on nosocomial enteric bacilli ß-lactamases producers. We analyzed 45 bacterial species, 36 to the Enterobacteriaceae family and nine of the other species of Gram-negative bacteria. The most prevalent species ESBL-producing was Klebsiella pneumoniaessp. pneumoniae (70% of isolates of this specie). The detection of ESBL was performed by phenotypic testing (approximation discs, combination discs and minimum inhibitory concentration - MIC - using E-test).

Results and conclusions

The trans-cinnamaldehyde showed antibacterial activity and promote inhibition of growth for all planktonic microorganisms ESBL positive tested, with MIC ranging between 0.95 mM and 1.90 mM. Bactericidal activity was detected at a concentration of 1.90 mM, regardless of the species analyzed in this study. Our results suggest that trans-cinnamaldehyde is a compound with potential antimicrobial against ESBL-producing bacteria and can be employed both in preventing infection through their application in solutions used in the processes of disinfection of hospital instruments and equipment but also in drug development for topical action.

Authors’ Affiliations

Faculty of Medicine of Sobral, Master's Program in Biotechnology, Federal University of Ceará (UFC), Campus-Sobral, Brazil
Faculty of Dentistry of Sobral, Federal University of Ceará (UFC), Campus-Sobral, Brazil
Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará (UFC), Fortaleza, Brazil
Faculty of Medicine of Sobral, Federal University of Ceará (UFC), Campus-Sobral, Brazil
Masters in Biotechnology, Federal University of Ceará (UFC), Campus-Sobral, Brazil


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© Pinto et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.