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BMC Proceedings

Open Access

The impact of endoplasmic reticulum stress on neutrophil function in alpha 1 antitrypsin deficiency

  • M Shaharom1,
  • H Kerr1,
  • D Bergin2 and
  • K Hurley2
BMC Proceedings20159(Suppl 1):A4

https://doi.org/10.1186/1753-6561-9-S1-A4

Published: 14 January 2015

Background

Alpha-1 antitrypsin (AAT) deficiency (AATD) is an autosomal recessive disorder characterized by reduced serum AAT levels. This results in little protection for the lower airways against destruction by proteolytic enzymes from neutrophils. The resulting chronic destruction of the lung, or emphysema, typically affects individuals with AATD in middle age and progresses slowly [1]. The ‘Z’ variant is the most common mutation held responsible for >95% of AATD cases. The Z mutation results in misfolding of the AAT protein leading to its accumulation in the endoplasmic reticulum (ER). In monocytes, the consequences of Z-AAT retention within the ER has been shown to activate the unfolded protein response (UPR) leading to proinflammatory cytokine production [2]. As neutrophils are the primary effector cell responsible for the pathological manifestations of AATD lung disease, the aim of this study was to determine whether the UPR occurs in neutrophils as a result of ER stress and the impact this pathway might have on the release proteolytic enzymes from these cells.

Methods

Neutrophils were isolated from both MM healthy controls and ZZ AATD patients. Cellular levels of ATF-6 (transcription factor) and GRP-78 (chaperone protein) which are markers of ER stress were quantified by Western blot. Purified MM neutrophils were cultured ± thapsigargin (1uM), a chemical inducer of ER stress. Cellular markers of ER stress were measured by Western blot. Degranulation level of MM neutrophils treated ± thapsigargin for 10 mins was determined by Western blot analysis of cell free supernatants using antibodies against MMP-9 (tertiary granules) and hCAP-18 (secondary granules). Statistical analysis was performed using GraphPad Prism 4.0. Ethical approval was obtained from the Beaumont Hospital ethic committee.

Results

Analysis of ATF-6 and GRP-78 protein expression revealed that ZZ-AATD neutrophils had an increase in the levels of these ER stress markers when compared to healthy control cells (P<0.05). Inducing ER stress in MM cells with thapsigargin also resulted in elevated ATF-6 and GRP-78 protein expression when compared to untreated cells. Furthermore, the treatment of MM neutrophils with thapsigargin resulted in a 10-fold and 4-fold increase in the release of MMP-9 and hCAP-18 respectively when compared to untreated MM-cells.

Conclusions

In conclusion, we document for the first time ER stress and the UPR response in ZZ-AATD neutrophils. Furthermore we demonstrate that ER stress and UPR can increase neutrophil degranulation. This shifts the paradigm of inflammation in AATD beyond lung and liver cells to include circulating immune cells.

Authors’ Affiliations

(1)
Royal College of Surgeons in Ireland
(2)
Respiratory Research Division, Beaumont Hospital

References

  1. Kelly E, et al: Alpha-1 antitrypsin deficiency. Respiratory Medicine CME. 2011, 4: 1-8. 10.1016/j.rmedc.2011.04.001.View ArticleGoogle Scholar
  2. Carroll TP, et al: Evidence for the Activation of the Unfolded Protein Response in Monocytes for Individuals with Alpha-1 Antitrypsin Deficiency. The Journal of Immunology. 2010, 184 (8): 4438-4546.View ArticleGoogle Scholar

Copyright

© Shaharom et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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