- Poster presentation
- Open Access
A drought stress transcriptome profiling as the first genomic resource for white teak - Gamhar - (Gmelina arborea Roxb) and related species
© Rosero et al; licensee BioMed Central Ltd. 2011
- Published: 13 September 2011
- Gene Ontology
- Drought Stress
- Drought Tolerance
- Water Deficit Stress
- Subtract cDNA Library
Gmelina (Gmelina arborea Roxb), also known as white teak is a tropical deciduous tree native from moist tropical forests of Asia. It has been successfully introduced in equatorial Africa, as well as central and south America. Gmelina is known for its very fast growing rate, its intrinsic disease, fire and drought tolerance, as well as the quality of its wood which is suitable for different types of uses such as paper pulping, plywood or particle board industry, furniture and light constructions. In addition, gmelina is considered a pioneer plant species capable of rapidly colonizing eroded or low nutritional quality lands, which makes it interesting for reforestation or landscape restoration programs. Despite its ecological and increasing economic importance, very little is known about the biology of this species and its remarkable field behavior such as drought tolerance, at the genetic, molecular and biochemical levels.
Genomic tools have recently increased the numbers and volume of genomic resources for several crop plants and trees and have contributed to enlarge our knowledge on basic aspects of plant biology, population dynamics or phylogeny; furthermore, they represent valuable sources of candidate genes and new molecular markers to assist breeding programs. Biological sequences reported to date in public databases and belonging to Gmelina do not exceed 20 entries: this very narrow availability of genetic information is the main bottleneck to initiate molecular breeding programs in Gmelina arborea.
We report here the first genomic resource for this tropical tree based on an RNAseq transcriptome profiling approach.
Two subtracted cDNA libraries were constructed from both leaf and root tissue of gmelina seedlings submitted to a water deficit stress. Subtracted cDNA sequencing was completed using 454 GS FLX Titanium sequencing. After sequence trimming, de novo assembly and clustering were completed using TGICL assembler and contigs were functionally annotated using BlastX against high quality curated protein reference databases. Both Gene Ontology (GO) terms and KEGG metabolic pathways assignments were used to further characterize transcripts. SSR motives were identified using MISA. Except for the KEGG pathway assignments, all the sequence analysis was compiled in the previously developed bioinformatic pipeline ESTTIK [http://esttik.cirad.fr/].
Summary of Gmelina arborea transcript sequencing and functional annotation
Size range (average) (bp)
Depth coverage (fold)
No Blast hi
GO annotated contigs
Contigs with Enzime Codes
KEGG pathway assigned contigs
Principal KEGG metabolic pathway assignments
Other Amino Acids
Cofactors and Vitamins
Terpenoids and Polyketides
Other Secondary Metabolites
The metabolic response in roots was slightly different with dominance of enzymes related to lipid, amino acid and carbohydrate metabolism, supporting important membrane and osmotic adjustment mechanisms in these tissues. Importantly, our gene catalog comprises many genes encoding proteins involved in signal perception and transduction, effector proteins as well as proteins with regulatory functions, allowing to cover the whole molecular response to drought stress. On the other hand the presence of several unigenes with no blast hit or with homology to unknown hypothetical proteins opens the possibility to uncover either previously unknown or less characterized protein functions related to drought stress.
Finally, we also identified microsatellite motifs within 428 unigenes, from which, 255 primer pairs have been designed for further experimental validation as functional SSR markers.
In this survey, we present the first unigene resource of this economically important tropical timber for which almost no prior genetic information existed. We identified drought stress related genes in different functional categories ranging from membrane bound sensor proteins, signal transduction proteins, transcription factors to metabolic or effector proteins. The SSR motifs found are good candidates for drought-related functional marker development in this species. Altogether, the results represent a first contribution to a better knowledge of the biology of white teak as well as the molecular mechanisms underlying its drought tolerance, which is essential to further encourage breeding program developments.
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.